Name: 硫酸脂酶
SKU: S1440758
Availability: InStock

硫酸脂酶

CAS: 9016-17-5 货号: S1440758 EC号: 232-772-1

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Sulfonation and sulfation are important processes in the metabolism of compounds such as hormones, neurotransmitters, and drugs. Sulfonation and sulfation are catalyzed by various sulfotransferases. In turn, desulfonation and desulfation occur via the action of sulfatase.

Several sulfatases occur in Helix pomatia (also known as Roman Snail). Early studies indicated the presence of at least two such sulfatases. One publication on two sulfatases isolated from Helix pomatia indicated molecular mass values of ~85 kDa by gel filtration chromatography. More recent work has postulated that Helix pomatia contains at least three sulfatases.

In vitro, this Helix pomatia sulfatase product has been used for deconjugation studies of various compounds, including:

• Environmental contaminants, such as bisphenol A

• Hirudin

• Vitamin E metabolites

• β-adrenoreceptor agonists

• Lignans from carob germ and carob seed

This product is a lyophilized powder. It is known to contain β-glucuronidase activity. For this reason, β-glucuronidase activity of this product is also determined.

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Enzymatic Assay of SULFATASE

PRINCIPLE:

CONDITIONS: T = 37°C, pH = 5.0, A515nm, Light path = 1 cm

METHOD: Colorimetric

REAGENTS:

A. 200 mM Sodium Acetate Buffer, pH 5.0 at 37°C(Prepare 50 ml in deionized water using Sodium Acetate, Trihydrate. Adjust to pH 5.0 at 37°C with 5 M HCl.)

B. 6.25 mM p-Nitrocatechol Sulfate Solution (PNCS)(Prepare 10 ml in deionized water using p-Nitrocatechol Sulfate, Dipotassium Salt.)

C. 1 M Sodium Hydroxide (NaOH)(Prepare 50 ml in deionized water using Sodium Hydroxide, Anhydrous.)

D. 0.2% (w/v) Sodium Chloride Solution(Prepare 50 ml in deionized water using Sodium Chloride.)

E. Sulfatase Enzyme Solution(Immediately before use, prepare a solution containing 2.5 - 5.0 units/ml of Sulfatase in cold Reagent D.)

PROCEDURE:

Pipette (in milliliters) the following reagents into suitable containers:

Test	Blank
Reagent A (Buffer)	0.50	0.50
Reagent B (PNCS)	0.40	0.40

Mix by swirling and equilibrate to 37°C. Then add:

Reagent E (Enzyme Solution)

0.10

------

Mix by swirling and incubate at 37°C for exactly 30 minutes. Then add:

Reagent C (NaOH)	5.00	5.00
Reagent E (Enzyme Solution)	------	0.10

Immediately mix by swirling. Transfer the solutions to suitable cuvettes and record the A515nm for both the Test and Blank using a suitable spectrophotometer.

CALCULATIONS:

2 = Time factor correction (Unit Definition

Time = 1 hour)

df = Dilution factor

6 = Total volume (in milliliters) of the assay

12.6 = Millimolar extinction coefficient of 4-nitrocatechol at 515 nm.

0.1 = Volume (in milliliter) of enzyme used

UNIT DEFINITION:

One unit will hydrolyze 1.0 µmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37°C.

FINAL ASSAY CONCENTRATION:

In a 1.00 ml reaction mix, the final concentrations are 100 mM sodium acetate, 2.5 mM p-nitrocatechol, 0.02% (w/v) sodium chloride, and 0.25 - 0.50 unit of sulfatase.

NOTES:

This enzyme assay is not to be used to assay Sulfatase from Aerobacter aerogenes.

规格

产品名称

硫酸脂酶

别名

芳基硫酸酯酶 | 苯硫酸酶 | β-葡萄糖醛酸酶/芳基硫酸酯酶 | 硫酸酯酶/β-葡萄糖醛酸酶, 芳基硫酸酯酶/β-葡萄糖醛酸酶

英文别名

规格或纯度

生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,来源于罗曼蜗牛; Sulfatase ≥10000 U/g enzyme powder; β-Glucuronidase ≥300000 U/g enzyme powder

生物活性

Sulfatase ≥10000 U/g enzyme powder; β-Glucuronidase ≥300000 U/g enzyme powder

种属

褐云玛瑙螺(Helix pomatia)

来源

天然

预测分子量

~85 kDa by gel filtration

CAS编号和信息

9016-17-5

酶学委员会编号

EC:3.1.6.1

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

来源于罗曼蜗牛; Sulfatase ≥10000 U/g enzyme powder; β-Glucuronidase ≥300000 U/g enzyme powder

复溶

Reconstitute in 0.2% (w/v) Sodium Chloride Solution.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃；复溶后建议分装，避免反复冻融。

单位定义

Sulfatase: One unit will hydrolyze 1.0 micromole ofP-Nitrocatechol Sulfate per hour at pH 5.0 at 37 Deg C. β-Glucuronidase: One unit will liberate 1.0 microgram ofPhenolphthalein from Phenolphthalein Glucuronide per hour at pH 5.0 at37 Deg C.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ26F0433804	分析证书	S1440758
ZJ26F0433805	分析证书	S1440758
ZJ26F0433806	分析证书	S1440758

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硫酸脂酶

储存与运输

质量文档

文献证明

概述

规格

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