磁微粒化学发光免疫分析是将磁性分离技术、化学发光技术、免疫分析技术三者结合的分析方法。磁微粒作为载体偶联抗体或抗原，可捕获目标分子，因其具有超顺磁性可实现多次磁分离，有利于检测仪器的全自动化并加快检测速度。目前，磁微粒化学免疫分析技术因其灵敏度高、检测范围宽、自动化程度高等优点已被广泛应用于生物医学检测领域。
链霉亲和素磁珠表面偶联有链霉亲和素，链霉亲和素（Streptavidin，SA）是由链霉菌分泌的一种蛋白质，由4条相同的肽链组成，其中每条肽链都能结合一个生物素，生物素与亲和素之间具有高亲合力以及多级放大效应目前被广泛应用于免疫检测中。
| 参数 | S1506723-1.2μm
| S1506723-2.8μm |
| 外观 | 红棕色悬浊液
| 红棕色悬浊液 |
| 固含量 | 10 mg/mL | 10 mg/mL |
| 粒径及分布 | 1.2± 0.1 μm, CV＜5%
| 2.8± 0.1 μm, CV＜5% |
| 游离生物素结合量 | 1200 - 2000 pmol/mg
| 800-1200 pmol/mg |
产品特点
1、弥散式结构：磁核材料为超顺磁性γ-Fe₂O₃纳米颗粒
2、氧化铁含量：12%-20%（不同磁珠有差异）
3、粒径：常规1.2、2.8μm
4、 粒径高度均一，批间差CV＜5%：保证稳定可靠的性能
5、分散性优良，检测本底低：自主创新设计的独特包覆层结构，具有极低的非特异性吸附
6、单批次2公斤级产能：满足化学发光客户日常需求
7、高稳定性：长时间存储后不会出现氧化变色和核磁泄露现象
使用说明
1、使用方案推荐
(1) 预混：将链霉亲和素磁珠与生物素（Biotin）偶联的抗原或抗体按照适当比例进行预混，通过 SA-Bio 形成磁珠偶联抗原或抗体复合物，作为检测试剂组分使用。
(2) 非预混：使用适当稀释液将链霉亲和素磁珠稀释后直接作为检测试剂组分使用，在检测过程中与生物素（Biotin） 偶联的抗原或抗体进行反应。
2、使用注意事项
(1) 使用链霉亲和素磁珠与生物素化抗体检测体系时需考虑检测样品中游离生物素干扰。
(2) 磁珠在取用前，务必充分振荡使磁珠呈均匀的悬浮状态。
(3) 干燥、冷冻操作极易引起磁珠团聚，且会影响磁珠表面功能基团的活性，请注意保存条件。
应用实例
1、 S1506723-1.2μm在游离甲状腺素（FT4）检测中与进口品牌J发光值对比
结论：
相同的磁珠偶联、试剂检测条件下，
S1506723-1.2μm磁珠在FT4检测中发光值与进口品牌J同类型1.5μm磁性微球基本一致；
S1506723-1.2μm三批发光值差异极小，生产工艺稳定批间差小。
2、S1506723-2.8μm在预混孕酮（Prog）检测中与进口品牌J发光值对比
Magnetic Particle Chemiluminescence Immunoassay is an analytical method that combines magnetic separation technology, chemiluminescence technology, and immunoassay technology. Magnetic particles, as carriers conjugated with antibodies or antigens, can capture target molecules. Due to their superparamagnetic properties, they enable multiple magnetic separations, facilitating full automation of detection instruments and accelerating testing speed. Currently, magnetic particle chemiluminescence immunoassay technology is widely used in biomedical detection due to its high sensitivity, broad detection range, and high automation.
Streptavidin Magnetic Beads are conjugated with streptavidin on their surface. Streptavidin (SA) is a protein secreted by Streptomyces, composed of four identical peptide chains, each capable of binding one biotin molecule. The high affinity and multilevel amplification effect between biotin and streptavidin are widely utilized in immunoassays.
Parameter
| S1506723-1.2μm
| S1506723-2.8μm |
Appearance
| Red-brown suspension
| Red-brown suspension
|
Solid Content
| 10 mg/mL | 10 mg/mL |
Particle Size & Distribution
| 1.15± 0.1 μm, CV＜5%
| 2.8± 0.1 μm, CV＜5% |
Free Biotin Binding Capacity
| 1200 - 2000 pmol/mg
| 800-1200 pmol/mg |
Product Features
Diffuse Structure: The magnetic core consists of superparamagnetic γ-Fe₂O₃ nanoparticles.
Iron Oxide Content: 12% - 20% (varies depending on the specific bead type).
Particle Size: Standard sizes of 1.2 μm and 2.8 μm.
Highly Uniform Particle Size with CV < 5% between Batches: Ensures stable and reliable performance.
Excellent Dispersion and Low Detection Background: Features a uniquely designed, proprietary coating layer that exhibits extremely low non-specific adsorption.
Production Capacity of 2 kg per Batch: Meets the routine demands of chemiluminescence customers.
High Stability: No oxidation/discoloration or magnetic core leakage occurs after long-term storage.
Instructions for Use
1. Recommended Usage Protocols
(1) Pre-mixing: Mix streptavidin magnetic beads with biotin-conjugated antigens or antibodies at an appropriate ratio to form bead-conjugated antigen/antibody complexes via SA-Biotin interaction, which can be used as a component of detection reagents.
(2) Non-pre-mixing: Dilute streptavidin magnetic beads with an appropriate diluent and use them directly as a component of detection reagents, allowing reaction with biotin-conjugated antigens or antibodies during the detection process.
2. Precautions
(1) When using streptavidin magnetic beads with biotinylated antibody detection systems, potential interference from free biotin in the sample must be considered.
(2) Before use, thoroughly vortex the magnetic beads to ensure a uniform suspension.
(3) Drying or freezing may cause bead aggregation and affect the activity of surface functional groups. Please adhere to recommended storage conditions.
Application Examples
1. Comparison of Luminescence Values Between S1506723-1.2μm and Imported Brand J in FT4 Detection
Conclusion:
Under identical bead conjugation and reagent detection conditions:
The luminescence values of S1506723-1.2μm magnetic beads in FT4 detection are essentially consistent with those of the similar 1.5μm magnetic microspheres from Imported Brand D.
The luminescence values across three batches of S1506723-1.2μm show minimal variation, indicating a stable production process and low batch-to-batch variation.
2. Comparison of Luminescence Values Between S1506723-2.8μm and Imported Brand J in Prog Detection
Conclusion:
Under identical bead conjugation and reagent detection conditions:
The luminescence values of S1506723-2.8μm magnetic beads in Prog detection are essentially consistent with those of the similar 3.0μm magnetic microspheres from Imported Brand J.
The luminescence values across three batches of S1506723-2.8μm show minimal variation, indicating a stable production process and low batch-to-batch variation.
ZJ26F0737573
危险品化学品经营许可证（带存储）