计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 3 篇同行评审文献引用。
概述
许多染料对DNA都有不同程度的亲和性，故可以作为染色体的染色。在染色体的常规染色中，一般用吉姆萨、地衣红、福尔根、石碳酸复红等可获得较好的染色效果。硫堇可用于口腔黏膜、尿液、羊水、绒毛细胞以及人工培养细胞等样本的染色体染色，尤其适用于较难上色的性染色质的染色，另外硫堇可用于尼氏小体、肥大细胞等物质的染色。
硫堇染色液（1%）主要由磷酸盐、硫堇组成，当染色尼氏体时尼氏体呈深蓝色，但染色肥大细胞时肥大细胞呈紫红色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备仪器和试剂
- PBS或生理盐水、蒸馏水、1mol/L HCl、固定液（甲醇:冰乙酸=3:1）
- 载玻片、盖玻片
- 显微镜
使用说明（仅供参考）
一、样本处理
1、口腔黏膜细胞
(1) 用PBS或生理盐水漱洗口腔数次，尽量去除口腔内细菌和其他杂物。
(2) 操作人员一手拉住患者的下唇，一手用压舌板或牙签钝头端刮取两侧颊部或下唇内侧的粘液，丢弃第一次刮取的细胞。
(3) 同一部位连续刮取数次，将刮取物涮入装有5ml生理盐水的离心管中。
(4) 1500g离心10-15min，弃上清液，留取细胞团。
(5) 加入新鲜固定液（甲醇:冰乙酸=3:1）10ml，轻轻混匀制成悬液，室温放置30min。
(6) 取一滴悬液至预冷的干净载玻片上，晾干。
2、尿液中脱落细胞
(1) 取患者干净的中段尿液，混匀，吸取10ml至离心管中。
(2) 1500-2000g离心10-15min，弃上清液，留取细胞团。
(3) 加入新鲜固定液（甲醇:冰乙酸=3:1）10ml，轻轻混匀制成悬液，室温放置30min。
(4) 1500g离心10-15min，弃上清液，留取细胞团。
(5) 根据细胞的多少，加入数滴新配制的固定液，充分混匀制成悬液。
(6) 取一滴悬液至预冷的干净载玻片上，晾干。
3、羊水细胞
(1) 按妇科常规经腹壁穿刺妊娠16周左右孕妇的羊水10ml至离心管中，抽取羊水时先抽取2-3ml丢弃，以免母体细胞的污染。
(2) 1500g离心10min，弃上清液，留取细胞团。
(3) 加入新鲜固定液（甲醇:冰乙酸=3:1）10ml，轻轻混匀制成悬液，室温放置30min。
(4) 1500g离心15min，弃上清液，留取细胞团。
(5) 根据细胞的多少，加入数滴新配制的固定液，充分混匀制成悬液。
(6) 取一滴悬液至预冷的干净载玻片上，晾干。
二、染色质染色
1、玻片标本置于1mol/L HCl中，37℃孵育20min。
2、蒸馏水充分冲洗，自然干燥。
3、将玻片样本浸入硫堇染色液（1%）染色15-20min。
4、蒸馏水冲洗，自然干燥。
5、在低倍镜下查找均匀分散的细胞群，转油镜认真观察。
染色结果
异染性物质呈紫红色，其他呈蓝色。
注意事项
1、在为了获得细胞沉淀的离心的过程中，对于特殊细胞，如果细胞沉淀不充分，可以适当提高离心力或延长离心时间。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、本产品仅用于科研，严禁他用。
Many dyes have varying degrees of affinity for DNA and can thus be used for chromosome staining. In routine chromosome staining, dyes such as Giemsa, orecin, Feulgen, and carbol fuchsin generally yield satisfactory results. Thionine can be used for chromosome staining in samples such as oral mucosa, urine, amniotic fluid, chorionic villus cells, and artificially cultured cells. It is particularly suitable for staining sex chromatin, which is often difficult to stain. Additionally, thionine can be used to stain substances such as Nissl bodies and mast cells.
Thionine Staining Solution (1%) is primarily composed of phosphate salts and thionine. When staining Nissl bodies, they appear deep blue, while mast cells appear purplish-red. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
- PBS or physiological saline, distilled water, 1 mol/L HCl, fixative solution (methanol:glacial acetic acid = 3:1)
- Glass slides, cover slips
- Microscope
Instructions for Use (For Reference Only)
I. Sample Processing
Oral Mucosal Cells
(1) Rinse the mouth several times with PBS or physiological saline to remove as many bacteria and debris as possible.
(2) The operator should hold the patient’s lower lip with one hand and use the blunt end of a tongue depressor or toothpick to scrape the mucosa from the inner cheek or lower lip. Discard the first scraping.
(3) Scrape the same area several times consecutively and rinse the scraped material into a centrifuge tube containing 5 mL of physiological saline.
(4) Centrifuge at 1500 × g for 10–15 minutes, discard the supernatant, and retain the cell pellet.
(5) Add 10 mL of fresh fixative solution (methanol:glacial acetic acid = 3:1), gently mix to form a suspension, and let stand at room temperature for 30 minutes.
(6) Place one drop of the suspension onto a pre-cooled, clean glass slide and air dry.
Exfoliated Cells in Urine
(1) Collect a clean midstream urine sample from the patient, mix well, and transfer 10 mL to a centrifuge tube.
(2) Centrifuge at 1500–2000 × g for 10–15 minutes, discard the supernatant, and retain the cell pellet.
(3) Add 10 mL of fresh fixative solution (methanol:glacial acetic acid = 3:1), gently mix to form a suspension, and let stand at room temperature for 30 minutes.
(4) Centrifuge at 1500 × g for 10–15 minutes, discard the supernatant, and retain the cell pellet.
(5) Depending on the number of cells, add a few drops of freshly prepared fixative solution and mix thoroughly to form a suspension.
(6) Place one drop of the suspension onto a pre-cooled, clean glass slide and air dry.
Amniotic Fluid Cells
(1) Using routine gynecological procedures, transabdominally aspirate approximately 10 mL of amniotic fluid from a pregnant woman at around 16 weeks of gestation into a centrifuge tube. Discard the first 2–3 mL of amniotic fluid to avoid contamination with maternal cells.
(2) Centrifuge at 1500 × g for 10 minutes, discard the supernatant, and retain the cell pellet.
(3) Add 10 mL of fresh fixative solution (methanol:glacial acetic acid = 3:1), gently mix to form a suspension, and let stand at room temperature for 30 minutes.
(4) Centrifuge at 1500 × g for 15 minutes, discard the supernatant, and retain the cell pellet.
(5) Depending on the number of cells, add a few drops of freshly prepared fixative solution and mix thoroughly to form a suspension.
(6) Place one drop of the suspension onto a pre-cooled, clean glass slide and air dry.
II. Chromatin Staining
Place the slide specimen in 1 mol/L HCl and incubate at 37°C for 20 minutes.
Rinse thoroughly with distilled water and air dry naturally.
Immerse the slide specimen in Thionine Staining Solution (1%) and stain for 15–20 minutes.
Rinse with distilled water and air dry naturally.
Under low magnification, locate evenly dispersed cell clusters, then switch to oil immersion for detailed observation.
Staining Results
Metachromatic substances appear purplish-red, while other structures appear blue.
Precautions
1. During centrifugation to obtain cell pellets, for certain cell types, if the pellet is insufficient, the centrifugation force or time may be appropriately increased.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. This product is for research use only. Any other use is strictly prohibited.
规格
别名
硫堇染液 | 硫堇水溶液
英文别名
Thionin Blue Staining Solution | Thionine Staining Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜,1%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Thionin Staining Solution (1%)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
EC号
676-854-9
分子类型
生物试剂/缓冲液
Isomeric SMILES
CC(=O)[O-].C1=CC2=C(C=C1N)SC3=CC(=[NH2+])C=CC3=N2
PubChem CID
分子量
287.34
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
安全和危险性（GHS）
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131781 |
分析证书 | S1508552 |
技术文档和文章
此产品的引用文献
溶液计算器
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