计算溶液所需的质量、体积或浓度。
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为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 3 篇同行评审文献引用。
概述
许多染料对DNA都有不同程度的亲和性，故可以作为染色体的染色。在染色体的常规染色中，一般用吉姆萨、地衣红、福尔根、石碳酸复红等可获得较好的染色效果。硫堇可用于口腔黏膜、尿液、羊水、绒毛细胞以及人工培养细胞等样本的染色体染色，尤其适用于较难上色的性染色质的染色，另外硫堇可用于尼氏小体、肥大细胞等物质的染色。
硫堇染色液（2%）主要由磷酸盐、硫堇组成，当染色尼氏体时尼氏体呈深蓝色，但染色肥大细胞时肥大细胞呈紫红色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
使用说明（仅供参考）
1、尼氏小体染色
- 固定：采用中性福尔马林固定液或其他固定液。
- 石蜡切片厚度6～8μm，常规二甲苯或脱蜡透明液脱蜡至蒸馏水。
- 将玻片样本浸入硫堇染色液（2%）染色10～15min，蒸馏水稍微冲洗。
- 95%乙醇迅速分化。
- 无水乙醇脱水，二甲苯或脱蜡透明液透明、中性树胶封固。
2、染色质染色
- 玻片标本置于1mol/L HCl中，37℃孵育20min。
- 蒸馏水充分冲洗，自然干燥。
- 将玻片样本浸入硫堇染色液（2%）染色10～15min。
- 蒸馏水冲洗，自然干燥。
- 在低倍镜下查找均匀分散的细胞群，转油镜认真观察。
染色结果
1、尼氏小体呈深蓝色，细胞核呈浅蓝色。
2、染色质呈紫红色，其他呈蓝色。
注意事项
1、用于尼氏体染色的组织要尽量新鲜，迅速固定，否则尼氏小体可能会溶解而不易着色。
2、尼氏小体标本应应避光保存，否则容易褪色。
3、在为了获得细胞沉淀的离心的过程中，对于特殊细胞，如果细胞沉淀不充分，可以适当提高离心力或延长离心时间。
4、该染色液比较适合尼氏小体的染色，虽然可以进行染色质染色，但不推荐。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Many dyes exhibit varying degrees of affinity for DNA and can therefore be used to stain chromosomes. For routine staining of chromosomes, good results are generally obtained with Giemsa, orecin, Feulgen, carbol fuchsin, etc. Thionine can be used for chromosome staining in samples such as oral mucosa, urine, amniotic fluid, chorionic villus cells, and cultured cells, and is particularly suitable for staining sex chromatin, which is often difficult to stain. Additionally, thionine can be used for staining structures such as Nissl bodies and mast cells.
Thionin Staining Solution (2%) is primarily composed of phosphate and thionine. When staining Nissl bodies, they appear dark blue; however, when staining mast cells, they appear purplish‑red. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Instructions for Use (For Reference Only)
1. Staining of Nissl Bodies
Fixation: Use neutral formalin fixative or other appropriate fixatives.
Paraffin sections 6–8 μm thick; routine deparaffinization with xylene or dewaxing clearing solution to distilled water.
Immerse the slide specimen in Thionine Staining Solution (2%) for 10–15 minutes; rinse briefly with distilled water.
Differentiate rapidly with 95% ethanol.
Dehydrate with absolute ethanol, clear with xylene or dewaxing clearing solution, and mount with neutral balsam.
2. Staining of Chromatin
Place the slide specimen in 1 mol/L HCl and incubate at 37°C for 20 minutes.
Rinse thoroughly with distilled water and air‑dry.
Immerse the slide specimen in Thionine Staining Solution (2%) for 10–15 minutes.
Rinse with distilled water and air‑dry.
Under low magnification, search for evenly dispersed cell populations, then carefully observe under oil immersion.
Staining Results
Nissl bodies appear dark blue; nuclei appear light blue.
Chromatin appears purplish‑red; other components appear blue.
Notes
Tissue intended for Nissl body staining should be as fresh as possible and fixed promptly; otherwise, Nissl bodies may dissolve and stain poorly.
Nissl body specimens should be stored protected from light, as they are prone to fading.
During centrifugation to obtain cell pellets, for certain cell types, if the pellet is insufficient, centrifugation force or time may be increased appropriately.
This staining solution is more suitable for Nissl body staining; although it can be used for chromatin staining, it is not recommended.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
硫堇染液 | 硫堇水溶液
英文别名
Thionin Blue Staining Solution | Thionine Staining Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜,2%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Thionin Staining Solution (2%)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
EC号
676-854-9
分子类型
生物试剂/缓冲液
Isomeric SMILES
CC(=O)[O-].C1=CC2=C(C=C1N)SC3=CC(=[NH2+])C=CC3=N2
PubChem CID
分子量
287.34
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
安全和危险性（GHS）
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131452 |
分析证书 | S1508553 |
此产品的引用文献
溶液计算器
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