STM溶液 (2M, pH7.4) 又称蔗糖-Tris-MgCl₂溶液,主要由2M蔗糖、10mM Tris、MgCl₂等组成,pH值约为7.4,过滤除菌,主要用于植物组织的根尖或表皮分离。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
研钵、离心机、纱布
操作步骤 (仅供参考):
1、选取根尖前部1-2mm的细胞,称取4g左右,洗净,切碎,置于研钵中,加入20ml 4℃预冷的STM溶液(2M, pH 7.4),研磨至匀浆。
2、纱布过滤,取滤液,4℃ 500g离心30s。
3、去上清,用少量STM溶液 (2M, pH 7.4)悬浮沉淀,悬浮液即为根尖分生组织细胞,如果第一次离心后沉淀中细胞碎片比较多,可重新悬浮细胞再,4℃ 500g离心30s。
注意事项:
1、注意无菌操作。
2、全过程尽量控制在4℃。
3、试剂开封后请尽快使用,以防影响后续实验效果。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Product Description
STM Solution (2M, pH 7.4), also known as Sucrose-Tris-MgCl₂ Solution, is mainly composed of 2 M sucrose, 10 mM Tris and MgCl₂, with a pH value of approximately 7.4. It is sterilized by filtration and primarily used for the isolation of root tips or epidermis from plant tissues. This reagent is for research use only and not intended for clinical diagnosis or any other purposes.
Materials to Be Prepared by Users
Mortar and pestle, centrifuge, gauze
Operating Procedures (For Reference Only)
1. Select the anterior 1-2 mm segment of root tips, weigh about 4 g of the sample, rinse it thoroughly, cut it into small pieces and place the pieces into a mortar. Add 20 ml of pre-chilled STM Solution (2M, pH 7.4) at 4 °C, and grind the mixture into homogenate.
2. Filter the homogenate through gauze, collect the filtrate, and centrifuge it at 500 × g for 30 seconds at 4°C.
3. Discard the supernatant, and resuspend the pellet in a small volume of STM Solution (2M, pH 7.4). The resulting suspension contains root tip meristematic cells. If the pellet contains a large amount of cell debris after the first centrifugation, resuspend the cells and centrifuge again at 500 × g for 30 seconds at 4°C.
Precautions
1. Ensure strict aseptic technique during operation.
2. Maintain the temperature at 4°C as much as possible throughout the entire process.
3. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.