计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
隐孢子虫卵囊染色检查方法有金胺-酚染色法、改良抗酸染色法等,金胺-酚染色液由金胺、酚等组成,适用于新鲜粪便样本或经福尔马林固定后的样本中隐孢子虫卵囊的染色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
| S1510269 | Component | 3×20 mL | Storage |
| S1510269A | 金胺酚染色液 | 20 mL | RT. Store in the dark. |
| S1510269B | 分化液 | 20 mL | RT. |
| S1510269C | 氧化剂 | 20 mL | RT. Store in the dark. |
操作步骤(仅供参考)
1、准备粪便标本薄涂片,自然晾干,甲醇中固定2~3min。
2、滴加金胺酚染色液于晾干的粪膜上,放置10~15min,水洗。
3、滴加分化液于粪膜上,放置1min,水洗。
4、滴加氧化剂于粪膜上,放置1min,水洗。
5、待干、荧光显微镜检查。
染色结果
低倍荧光显微镜下,卵囊呈一圆形小亮点,发出乳白色荧光;
高倍荧光显微镜下,卵囊呈乳白色或略带绿色,卵囊壁为一薄层,多数卵囊周围深染,中央淡然,呈环状。
注意事项
1、有些标本可出现非特异性的荧光颗粒,注意加以区别。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Staining methods for the detection of Cryptosporidium oocysts include the auramine-phenol staining method and the modified acid-fast staining method, among others. The Auramine-Phenol Staining Solution is composed of auramine and phenol, and is suitable for staining Cryptosporidium oocysts in fresh fecal specimens or specimens fixed with formalin. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
| S1510269 | Component | 3×20 mL | Storage |
| S1510269A | Auramine-Phenol Staining Solution | 20 mL | RT. Store in the dark. |
| S1510269B | Differentiation Solution | 20 mL | RT. |
| S1510269C | Oxidizing Solution | 20 mL | RT. Store in the dark. |
Operating Steps (For Reference Only)
Prepare a thin fecal smear on a glass slide and allow it to air dry. Fix with methanol for 2–3 minutes.
Apply Auramine-Phenol Staining Solution onto the air-dried fecal film and let stand for 10–15 minutes. Rinse with water.
Apply Differentiation Solution onto the fecal film and let stand for 1 minute. Rinse with water.
Apply Oxidizing Solution onto the fecal film and let stand for 1 minute. Rinse with water.
Allow the slide to dry and examine under a fluorescence microscope.
Staining Results
Under low magnification fluorescence microscopy, oocysts appear as small, round bright spots emitting a milky white fluorescence.
Under high magnification fluorescence microscopy, oocysts appear milky white or slightly greenish. The oocyst wall is thin, and most oocysts show a deeply stained periphery with a paler center, giving a ring‑like appearance.
Precautions
Some specimens may show non‑specific fluorescent particles; careful differentiation is required.
For your safety and health, please wear a lab coat and disposable gloves during operation.
Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1510269 |
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