枸橼酸钠又称柠檬酸钠，其抗凝原理是通过与血液中的钙离子（Ca²⁺）结合，形成稳定的可溶性螯合物，从而有效移除参与凝血级联反应的关键钙离子，达到阻止血液凝固的目的。本产品适用于凝血功能检测：如凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）等凝血象的测定；红细胞沉降率（ESR）测定：特别适用于魏氏法（Westergren Method） 进行血沉测定。
使用说明
使用前请确保充分了解实验方案，并严格遵守无菌操作规范。
1. 常规抗凝比例（通用）
- 推荐抗凝剂与全血的体积比为 1:9。
- 操作示例：取 0.2 mL 本品，加入 1.8 mL 新鲜全血，轻轻颠倒混匀 5-8 次，避免剧烈震荡。
2. 魏氏法血沉测定专用比例
注意事项
1. 使用限制：本品为科研专用试剂，严禁用于临床诊断、输血或人体治疗。
2. 比例精确性：抗凝比例至关重要，比例不当会直接影响实验结果（如血凝检测结果假性延长或缩短）。请使用精确的移液器具。
3. 样本处理：血液加入后须立即轻柔混匀。避免样本产生泡沫或溶血。
4. 时效性：抗凝后的血液样本建议在2小时内完成检测，若需保存，应遵循具体实验的指南（通常 2-8°C 短期保存）。
5. 个人防护：操作时请穿实验服，佩戴一次性手套和必要的眼部防护装备。如接触皮肤或眼睛，请立即用大量清水冲洗。
6. 废弃物处理：所有接触过血液的耗材、剩余样本及本品溶液，均应作为生物危害性废弃物处理，并遵守所在机构的生物安全规定。
Sodium citrate, also known as trisodium citrate, functions as an anticoagulant by binding to calcium ions (Ca²⁺) in the blood, forming stable soluble chelate complexes. This effectively removes the key calcium ions involved in the coagulation cascade, thereby preventing blood clotting. This product is suitable for:
Coagulation function testing: such as the determination of prothrombin time (PT), activated partial thromboplastin time (APTT), and other coagulation parameters.
Erythrocyte sedimentation rate (ESR) determination: particularly suitable for ESR measurement using the Westergren Method.
Usage Instructions
Please ensure a thorough understanding of the experimental protocol and strictly adhere to aseptic techniques before use.
1. General Anticoagulation Ratio (Universal)
The recommended volume ratio of anticoagulant to whole blood is 1:9.
Example: Take 0.2 mL of this product, add 1.8 mL of fresh whole blood, and gently invert the tube 5-8 times to mix. Avoid vigorous shaking.
2. Special Ratio for Westergren Method ESR Determination
The recommended volume ratio of anticoagulant to whole blood is 1:4.
Example: Take 0.4 mL of this product, add 1.6 mL of fresh whole blood, and mix as described above.
Precautions
1. Intended Use: This product is for research use only. It is strictly prohibited for clinical diagnosis, blood transfusion, or human treatment.
2. Ratio Accuracy: The anticoagulant-to-blood ratio is critical. An incorrect ratio will directly affect experimental results (e.g., causing falsely prolonged or shortened coagulation times). Use precise pipetting equipment.
3. Sample Handling: Blood samples must be mixed gently immediately after collection. Avoid foaming or hemolysis.
4. Timeliness: It is recommended to complete testing of anticoagulated blood samples within 2 hours. If storage is necessary, follow the specific guidelines for your experiment (typically short-term storage at 2-8°C).
5. Personal Protection: Wear a lab coat, disposable gloves, and necessary eye protection during operation. If contact with skin or eyes occurs, rinse immediately with plenty of water.
6. Waste Disposal: All consumables that have contacted blood, residual samples, and solutions of this product must be treated as biohazardous waste, in compliance with your institution's biosafety regulations.
ZJ26F0736958
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