计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
超氧阴离子(O₂•⁻)是通过将电子加到氧上而产生的一种短暂的自由基。它是由紫外线、香烟烟雾,环境污染物和γ辐射等环境因素作用形成的,或者是由黄嘌呤氧化酶或 NADPH 氧化酶等氧化酶衍生而来的。O₂•⁻一旦形成,就会攻击细胞成分并破坏脂质,蛋白质和 DNA。这会引发多种疾病,包括癌症,动脉粥样硬化,类风湿性关节炎,糖尿病,肝损害和中枢神经系统疾病。清除超氧阴离子自由基的研究越来越受到重视。 本试剂盒检测原理是黄嘌呤氧化酶(XO)催化反应提供超氧阴离子(O₂•⁻),O₂•⁻与四唑盐WST-8染料反应形成水溶性的有色甲臜产物,可以很容易地进行定量。样品清除了O₂•⁻,因此用于显色反应的O₂•⁻较少。通过比色法在 450 nm 下测量样品的这种清除能力。
Superoxide anion (O₂•⁻) is a short-lived free radical generated by the addition of an electron to molecular oxygen. It is formed through the action of environmental factors such as ultraviolet light, cigarette smoke, environmental pollutants, and gamma radiation, or is derived from oxidases like xanthine oxidase or NADPH oxidase. Once formed, O₂•⁻ attacks cellular components, damaging lipids, proteins, and DNA. This process can trigger various diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. Consequently, research on scavenging superoxide anion radicals is receiving increasing attention. The detection principle of this kit is as follows: xanthine oxidase (XO) catalyzes a reaction that provides a source of superoxide anion (O₂•⁻). O₂•⁻ reacts with the tetrazolium salt WST-8 dye to form a water-soluble, colored formazan product, which can be easily quantified. Samples that scavenge O₂•⁻ result in less O₂•⁻ available for the chromogenic reaction. This scavenging capacity of the sample is measured colorimetrically at 450 nm.