计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖是源(叶片等)光合产物向“库”器官运输的主要形态。蔗糖合成酶(Sucrose Synthase, EC 2.4.1.13)是双向反应酶,既可催化蔗糖合成又可催化蔗糖分解,是蔗糖代谢的关键酶之一。研究其分解方向 SS-I 的活性对于植物蔗糖降解以及淀粉合成具有重要意义。该试剂盒提供了一种简单、方便、快速的蔗糖合成酶(SS-I)活性检测方法,适用于植物组织样本。其原理是 SS-I 催化蔗糖和 UDP 生成游离果糖和 UDPG,采用 3, 5-二硝基水杨酸法测定还原糖的含量来反映酶活性的高低。
Sucrose is the primary transport form of photosynthetic products from source organs (e.g., leaves) to sink organs. Sucrose Synthase (SS, EC 2.4.1.13) is a bidirectional (reversible) enzyme that catalyzes both sucrose synthesis and degradation, making it a key enzyme in sucrose metabolism. Studying the activity of its degradative direction (SS-I) is of significant importance for understanding sucrose breakdown and starch synthesis in plants. This assay kit provides a simple, convenient, and rapid method for measuring sucrose synthase (SS-I) activity in plant tissue samples. The detection principle is as follows: SS-I catalyzes the reaction of sucrose with UDP to produce free fructose and UDP-glucose (UDPG). The generated reducing sugar (fructose) is then quantified using the 3,5-dinitrosalicylic acid (DNS) method, which reflects the level of enzyme activity.