计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖是源(叶片等)光合产物向“库”器官运输的主要形态。蔗糖合成酶(Sucrose Synthase, EC2.4.1.13)是双向反应酶,既可催化蔗糖合成又可催化蔗糖分解,是蔗糖代谢的关键酶之一。研究其合成方向 SS-II 的活性对于植物蔗糖合成具有重要意义。该试剂盒能够检测植物组织中蔗糖合成酶活性,其原理是SS-II 催化游离果糖与葡萄糖供体 UDPG 反应生成蔗糖,蔗糖与间苯二酚反应可呈现颜色变化,在 480 nm 下有特征吸收峰,酶活力大小与颜色的深浅成正比。
Sucrose is the primary transport form of photosynthetic products from source organs (such as leaves) to sink organs. Sucrose Synthase (SS, EC 2.4.1.13) is a bidirectional (reversible) enzyme that catalyzes both sucrose synthesis and degradation, making it a key enzyme in sucrose metabolism. Studying the activity of its synthetic direction (SS-II) is of significant importance for understanding sucrose synthesis in plants. This assay kit is designed to measure sucrose synthase activity in plant tissues. The detection principle is as follows: SS-II catalyzes the reaction between free fructose and the glucose donor UDP-glucose (UDPG) to produce sucrose. The generated sucrose then reacts with resorcinol, resulting in a color change. This colored product exhibits a characteristic absorption peak at 480 nm. The enzyme activity is proportional to the intensity of the color developed.