计算溶液所需的质量、体积或浓度。
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SSC 缓冲液(Saline Sodium Citrate Buffer)主要由氯化钠、柠檬酸钠等组成,适用于各类核酸杂交实验,包括 RNA 杂交(Northern 印迹)、DNA 杂交(Southern 印迹)等。
SSC 缓冲液 (20×, pH 7.4) 为浓缩缓冲液,pH 值约 7.4,需稀释至 1× 工作液后使用,是核酸杂交实验的常用缓冲体系。
本试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
根据实验需求,取适量 SSC 缓冲液 (20×, pH 7.4),用去离子水或蒸馏水稀释至 1× 后即可使用。
注意事项
1. 若单次使用量较小,建议将试剂分装后再使用,降低污染与反复冻融风险。
2. 操作时请穿戴实验服及一次性手套,保证实验安全。
3. 试剂开封后请尽快使用并密封保存,以免影响后续实验效果。
SSC Buffer is primarily composed of sodium chloride and sodium citrate. It is suitable for various nucleic acid hybridization experiments, including RNA hybridization (Northern blot) and DNA hybridization (Southern blot).
SSC Buffer (20×, pH 7.4) is a concentrated buffer with a pH of approximately 7.4. It should be diluted to a 1× working solution before use and is a commonly used buffer system in nucleic acid hybridization experiments.
This reagent is for research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
According to experimental requirements, take an appropriate amount of SSC Buffer (20×, pH 7.4) and dilute to 1× with deionized water or distilled water before use.
Notes
If the single-use volume is small, it is recommended to aliquot the reagent before use to reduce the risk of contamination and repeated freezing and thawing.
Please wear laboratory appropriate attire and disposable gloves during operation to ensure experimental safety.
Use the reagent as soon as possible after opening and keep it sealed to avoid affecting subsequent experimental results.
| pH | 7.4 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1518778 |
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