Amylase (EC 3.2.1.1) is a general term for a class of enzymes that catalyze the hydrolysis of starch. Amylase in soil is mainly derived from microorganisms and is an important enzyme preparation widely used in grain processing, food, brewing, fermentation, textile industry, and pharmaceutical industry.
Determination principle: Amylase hydrolyzes starch to produce reducing sugars, which react with 3,5-dinitrosalicylic acid to form a reddish-brown substance with a characteristic absorption peak at 508 nm. The color intensity is proportional to the amount of reducing sugars within a certain range.