计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤蔗糖酶(S-SC)能够水解蔗糖变成相应的单糖而被机体吸收,其酶促作用产物与土壤中有机质、氮、磷含量,微生物数量及土壤呼吸强度密切关,是评价土壤肥力的重要指标。本试剂盒提供了一种简单、方便、快速的蔗糖酶活性检测方法,适用于土壤样本。其原理是 S-SC 催化蔗糖降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在 540 nm 有特征光吸收,在一定范围内 540 nm 光吸收增加速率与 S-SC 活性成正比。
Soil sucrase (S-SC) hydrolyzes sucrose into corresponding monosaccharides for uptake by organisms. The products of its enzymatic reaction are closely related to soil organic matter, nitrogen and phosphorus content, microbial population, and soil respiration intensity, making it an important indicator for assessing soil fertility. This kit provides a simple, convenient, and rapid method for detecting sucrase activity, suitable for soil samples. The principle is based on S-SC catalyzing the degradation of sucrose to produce reducing sugars, which then react with 3,5-dinitrosalicylic acid to form a reddish-brown amino compound. This compound exhibits characteristic light absorption at 540 nm, and within a certain range, the rate of increase in absorbance at 540 nm is proportional to S-SC activity.