BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖不仅是重要的光合产物，也是植物体内运输的主要物质，还是碳水化合物的贮存形式之一。蔗糖磷酸合成酶（SPS, EC2.4.1.14）以果糖-6-磷酸为受体，形成的蔗糖磷酸在蔗糖磷酸酶的作用下形成蔗糖。一般把蔗糖磷酸酯合成酶-蔗糖磷酸酶系统看作是蔗糖合成的主要途径。本试剂盒提供了一种简单、方便、快速的SPS活性检测方法，适用于植物组织样本。其原理是蔗糖磷酸合成酶催化果糖-6-磷酸形成蔗糖磷酸，蔗糖磷酸与间苯二酚反应可呈现颜色变化，在 480 nm 下有特征吸收峰，酶活力大小与颜色的深浅成正比。
Sucrose is not only a key photosynthetic product but also the primary transport substance in plants and one of the storage forms of carbohydrates. Sucrose phosphate synthase (SPS, EC 2.4.1.14) uses fructose-6-phosphate as an acceptor to form sucrose phosphate, which is then converted into sucrose by sucrose phosphatase. The sucrose phosphate synthase–sucrose phosphatase system is generally regarded as the main pathway for sucrose synthesis. This kit provides a simple, convenient, and rapid method for detecting SPS activity, suitable for plant tissue samples. The principle is based on sucrose phosphate synthase catalyzing the formation of sucrose phosphate from fructose-6-phosphate. Sucrose phosphate reacts with resorcinol to produce a color change, which exhibits a characteristic absorption peak at 480 nm. The enzyme activity is proportional to the intensity of the color developed.
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