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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
天冬酰胺酶（ASNase，EC 3.5.1.1）是酰胺酶的一种，催化天冬酰胺水解成天冬氨酸和氨，在氮素代谢中具有重要调控作用。 土壤中的天冬酰胺酶（S-ASNase）催化天冬酰胺水解成天冬氨酸和氨，利用氨在强碱的环境下与次氯酸盐和苯酚作用，生成水溶性染料靛酚蓝，溶液颜色稳定。其在630nm处有特征吸收峰，通过检测氨增加的速率，即可计算该酶活性大小。
Asparaginase (ASNase, EC 3.5.1.1) is a type of amidase that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia, playing an important regulatory role in nitrogen metabolism. Soil Asparaginase (S-ASNase) catalyzes the hydrolysis of asparagine in the soil to produce aspartic acid and ammonia. Under strong alkaline conditions, the ammonia reacts with hypochlorite and phenol to form a stable, water-soluble blue indophenol compound. This compound has a characteristic absorption peak at 630 nm, and the enzyme activity can be calculated by measuring the rate of increase in ammonia production.
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