计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖转化酶(Invertase,Ivr)催化蔗糖不可逆地分解为果糖和葡萄糖,是高等植物蔗糖代谢关键酶之一。根据最适pH,将Ivr 分为酸性转化酶(Acid invertase,AI)和中性转化酶(Neutral invertase,NI)两种类型。本试剂盒提供了一种简单、方便、快速的土壤中性转化酶活性检测方法,适用于土壤样本。其原理是S-NI 催化蔗糖分解产生还原糖,进一步与 3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在 540 nm 有特征光吸收,在一定范围内540nm光吸收值与还原糖生成量成正比。通过光吸收增加速率来计算 S-NI 活性。
Sucrose invertase (Invertase, Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose and is one of the key enzymes in sucrose metabolism in higher plants. Based on its optimal pH, Ivr is classified into two types: acid invertase (AI) and neutral invertase (NI). This kit provides a simple, convenient, and rapid method for detecting soil neutral invertase (S-NI) activity, suitable for soil samples. The principle is that S-NI catalyzes the decomposition of sucrose to produce reducing sugars, which further react with 3,5-dinitrosalicylic acid to form a reddish-brown amino compound. This compound exhibits characteristic light absorption at 540 nm, and within a certain range, the absorbance at 540 nm is proportional to the amount of reducing sugars generated. The activity of S-NI is calculated based on the rate of increase in light absorption.