BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖转化酶（Invertase，Ivr）催化蔗糖不可逆地分解为果糖和葡萄糖，是高等植物蔗糖代谢关键酶之一。根据最适pH，将高等植物 Ivr 分为酸性转化酶（Acid invertase，AI）和中性转化酶（Neutral invertase，NI）两种类型。AI 的最适pH 为3~5。AI 分为可溶性 AI(S-AI)和细胞壁不溶性 AI（B-AI）两种类型。S-AI 主要存在于细胞液泡或自由空间中，最适 pH 为4.5~5.0（酸性），通过降解液泡中蔗糖，调节液泡中蔗糖的利用和果实内糖类的积累。本试剂盒可检测植物组织样本。在该试剂盒中，S-AI 催化蔗糖降解产生还原糖，进一步与 3,5－二硝基水杨酸反应，生成棕红色氨基化合物，在510 nm 有特征光吸收，在一定范围内 510 nm 光吸收增加速率与 S-AI 活性成正比。
Sucrose invertase (Invertase, Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose and is one of the key enzymes in sucrose metabolism in higher plants. Based on their optimal pH, Ivr in higher plants is classified into two types: acid invertase (AI) and neutral invertase (NI). The optimal pH for AI ranges from 3 to 5. AI is further divided into two forms: soluble AI (S-AI) and cell wall-bound insoluble AI (B-AI). S-AI is mainly located in the cell vacuole or free space, with an optimal pH of 4.5–5.0 (acidic). It regulates sucrose utilization in the vacuole and sugar accumulation in fruits by degrading vacuolar sucrose. This kit is suitable for detecting S-AI activity in plant tissue samples. In the assay, S-AI catalyzes the degradation of sucrose to produce reducing sugars, which then react with 3,5-dinitrosalicylic acid to form a reddish-brown amino compound. This compound exhibits characteristic light absorption at 510 nm, and within a certain range, the rate of increase in absorbance at 510 nm is proportional to S-AI activity.
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