BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖转化酶（Invertase，Ivr）催化蔗糖不可逆地分解为果糖和葡萄糖，是高等植物蔗糖代谢关键酶之一。根据最适 pH，Ivr分为酸性转化酶（AI）和中性转化酶（NI）两种类型。AI的最适pH为3～5。AI分为可溶性AI(S-AI)和细胞壁不溶性AI（B-AI）两种类型。S-AI主要存在于细胞液泡或自由空间中，最适 pH 为4.5~5.0（酸性），通过降解液泡中蔗糖，调节液泡中蔗糖的利用和果实内糖类的积累。 测定原理：S-AI催化蔗糖降解产生还原糖，进一步与3,5－二硝基水杨酸反应，生成棕红色氨基化合物，在510nm有特征光吸收，在一定范围内510nm光吸收增加速率与S-AI活性成正比。
Sucrose invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose and is one of the key enzymes in sucrose metabolism in higher plants. Based on optimal pH, Ivr is divided into two types: acid invertase (AI) and neutral invertase (NI). AI has an optimal pH of 3–5. AI is further divided into soluble AI (S-AI) and cell wall‑bound insoluble AI (B-AI). S-AI is mainly present in the cell vacuole or free space, with an optimal pH of 4.5–5.0 (acidic). It regulates the utilization of sucrose in the vacuole and the accumulation of sugars in fruits by degrading vacuolar sucrose. Determination principle: S-AI catalyzes the degradation of sucrose to produce reducing sugars, which further react with 3,5‑dinitrosalicylic acid to form a reddish‑brown amino compound with characteristic light absorption at 510 nm. Within a certain range, the rate of increase in absorbance at 510 nm is proportional to S-AI activity.
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