计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
S-AI 在 pH 为 4.5-5.0(酸性)条件下催化蔗糖不可逆地分解为果糖和葡萄糖,是土壤微生物蔗糖代谢关键酶之一。在该试剂盒中,S-AI 催化蔗糖降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在 510 nm 有特征光吸收,在一定范围内 510 nm 光吸收增加速率与S-AI 活性成正比。
S-AI irreversibly cleaves sucrose into fructose and glucose under acidic conditions (pH 4.5–5.0) and is one of the key enzymes in sucrose metabolism for soil microorganisms. In this kit, S-AI catalyzes the degradation of sucrose to produce reducing sugars, which further react with 3,5-dinitrosalicylic acid to form a brownish-red amino compound. This compound exhibits characteristic light absorption at 510 nm, and within a certain range, the rate of increase in absorbance at 510 nm is proportional to S-AI activity.