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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
S-AI在pH 为4.5~5.0(酸性)条件下催化蔗糖不可逆地分解为果糖和葡萄糖,是土壤微生物蔗糖代谢关键酶之一。 测定原理:S-AI催化蔗糖降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在510nm有特征光吸收,在一定范围内510nm光吸收增加速率与AI活性成正比。
S-AI catalyzes the irreversible decomposition of sucrose into fructose and glucose under acidic conditions (pH 4.5–5.0) and is one of the key enzymes in sucrose metabolism in soil microorganisms. Determination principle: S-AI catalyzes the degradation of sucrose to produce reducing sugars, which further react with 3,5‑dinitrosalicylic acid to form a reddish‑brown amino compound with characteristic light absorption at 510 nm. Within a certain range, the rate of increase in absorbance at 510 nm is proportional to AI activity.