Sorbitol dehydrogenase (SDH, EC 1.1.1.14) catalyzes the dehydrogenation of sorbitol to produce fructose, and is one of the key enzymes regulating sorbitol content in organisms.
The detection principle of this kit is based on the reaction where SDH catalyzes the dehydrogenation of sorbitol to form fructose, while simultaneously reducing NAD⁺ to generate NADH. The rate of increase in absorbance at 340 nm is measured to calculate SDH activity.
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