计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
可溶性淀粉合成酶(Soluble starch synthase,SSS,EC 2.4.1.21)通常以游离态存在于质体基质中,催化淀粉链延长,主要负责支链淀粉的合成。该产品可检测植物组织样本。在该试剂盒中,SSS催化 ADPG 与淀粉引物(葡聚糖)反应,将葡萄糖分子转移到淀粉引物上,同时生成 ADP;进一步通过反应体系中添加的丙酮酸激酶、己糖激酶和 6-磷酸葡萄糖脱氢酶依次催化NADP⁺还原为NADPH,其中 NADPH 生成量与前一步反应生成的ADP数量呈正比,通过 340 nm 下测定 NADPH 的增加量,可以计算 SSS 活性。
Soluble starch synthase (SSS, EC 2.4.1.21) usually exists in a free state within the plastid stroma. It catalyzes the elongation of starch chains and is primarily responsible for the synthesis of amylopectin. This product is suitable for detecting plant tissue samples. In this kit, SSS catalyzes the reaction between ADPG and a starch primer (glucan), transferring a glucose molecule to the starch primer while simultaneously generating ADP. Subsequently, through the sequential action of pyruvate kinase, hexokinase, and glucose-6-phosphate dehydrogenase added to the reaction system, NADP⁺ is reduced to NADPH. The amount of NADPH generated is proportional to the amount of ADP produced in the preceding reaction. By measuring the increase in NADPH at 340 nm, SSS activity can be calculated.