计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
SSS(EC 2.4.1.21)通常以游离态存在于质体基质中,催化淀粉链延长,主要负责支链淀粉的合成。 测定原理:SSS催化ADPG与淀粉引物(葡聚糖)反应,将葡萄糖分子转移到淀粉引物上,同时生成ADP,在反应体系中添加的丙酮酸激酶、己糖激酶和6-磷酸葡萄糖脱氢酶依次催化NADP+还原为NADPH,NADPH生成量与前一步反应中ADP生成量呈正比,340nm下测定NADPH增加量即可计算SSS活性。
SSS (EC 2.4.1.21) generally exists in a free form in the plastid stroma, catalyzes the elongation of starch chains, and is primarily responsible for amylopectin synthesis. Determination Principle: SSS catalyzes the reaction between ADPG and a starch primer (glucan), transferring glucose molecules to the starch primer while generating ADP. Pyruvate kinase, hexokinase, and glucose-6-phosphate dehydrogenase added to the reaction system sequentially catalyze the reduction of NADP⁺ to NADPH. The amount of NADPH produced is proportional to the amount of ADP generated in the preceding reaction. SSS activity can be calculated by measuring the increase in NADPH at 340 nm.