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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤羟胺还原酶能将土壤中氮代谢过程中形成的中间产物羟胺还原为氨，土壤中的还原态化合物可作为氢的供体，其强弱影响到土壤氮代谢过程中氮素的氨挥发损失，间接影响氮肥的利用效率。 测定原理：硫酸铁铵中的Fe³⁺可将羟胺氧化为氮气，自身被还原为Fe²⁺，Fe²⁺在弱酸条件下与邻菲罗琳形成橙红色配合物，在510nm处有吸收峰，羟胺还原酶作用于羟胺，使配合物形成量减少，510nm处吸光值的减少可反映羟胺还原酶的活性。
Soil hydroxylamine reductase can reduce hydroxylamine, an intermediate product formed during nitrogen metabolism in soil, to ammonia. Reduced compounds in the soil can serve as hydrogen donors, and their availability affects the loss of ammonia through volatilization during soil nitrogen metabolism, thereby indirectly influencing the efficiency of nitrogen fertilizer utilization. Determination Principle: Fe³⁺ in ferric ammonium sulfate can oxidize hydroxylamine to nitrogen gas while being reduced to Fe²⁺. Under weak acid conditions, Fe²⁺ forms an orange-red complex with o-phenanthroline, which exhibits an absorption peak at 510 nm. Hydroxylamine reductase acts on hydroxylamine, reducing the amount of complex formed. The decrease in absorbance at 510 nm reflects the activity of hydroxylamine reductase.
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