核酸是土壤有机磷的组分之一，在核酸酶的作用下，核酸经过多步分解最终释放出无机磷，水解产物是植物的磷源之一。土壤核酸酶的活性通过度量土壤核苷酸酶的活性测得。土壤核酸酶即土壤核苷酸酶通过水解底物核糖核苷酸生成无机磷和其他物质，无机磷在酸性环境中与钼酸铵反应生成蓝色复合物，通过在 700nm 处检测该有色物质的生成速率即可计算土壤核酸酶活性大小。
Nucleic acids are a component of soil organic phosphorus. Under the action of nucleases, nucleic acids undergo multi-step decomposition and ultimately release inorganic phosphorus, and the hydrolysis products serve as one of the phosphorus sources for plants. The activity of soil nucleases is determined by measuring the activity of soil nucleotidases. Soil nucleotidases hydrolyze ribonucleotides to produce inorganic phosphorus and other substances. In an acidic environment, inorganic phosphorus reacts with ammonium molybdate to form a blue complex. The soil nuclease activity is calculated by measuring the rate of formation of this colored product at 700 nm.
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