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α-木糖苷酶(EC 3.2.1.177)是一类木聚糖降解水解酶，存在于微生物等生物体，促使非还原末端α-D-木糖残基的水解，释放出α-D-木糖。 土壤中α-木糖苷酶催化对硝基苯酚-α-D-木糖苷产生对硝基苯酚（PNP），该产物在405nm处有特征吸收峰，通过测定 405nm 光吸收增加速率，即可计算土壤α-木糖苷酶活性。
α-Xylosidase (EC 3.2.1.177) is a xylan-degrading hydrolase found in microorganisms and other organisms. It catalyzes the hydrolysis of non-reducing terminal α-D-xylose residues, releasing α-D-xylose. Assay principle: In soil, α-xylosidase catalyzes the reaction of p-nitrophenyl-α-D-xyloside to produce p-nitrophenol (PNP), which exhibits a characteristic absorption peak at 405 nm. The soil α-xylosidase activity is calculated by measuring the rate of increase in absorbance at 405 nm.
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