BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤有机碳（Soilorganiccarbon, SOC）是指通过微生物作用所形成的腐殖质、动植物残体和微生物体中的碳元素含量，即为土壤有机碳(SOC)。土壤有机碳根据微生物可利用程度分为易分解有机碳，难分解有机碳和惰性有机碳。易分解者有较高的生物利用率与损失率，难分解者则有较高的残留率，一般占土壤有机质的60%-80%。且有相当多的部分参加到腐殖质的形成作用中去。土壤有机碳 (SOC) 含量检测试剂盒 (微量法)可检测土壤样本。在该试剂盒中，在加热条件下，土壤样品中的有机碳被过量重铬酸钾-硫酸溶液氧化，重铬酸钾中的六价铬(Cr⁶⁺)被还原为三价铬(Cr³⁺)，其含量与样品中有机碳的含量成正比，于585nm波长处测定吸光度，根据三价铬(Cr³⁺)的含量计算有机碳含量。
组分与储存：
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自备耗材
·酶标仪或可见光分光光度计（能测585nm处的吸光度）
·96孔板或微量玻璃比色皿、可调节式移液枪及枪头、1.5mL离心管
·水浴锅、离心机、消解仪、消解管、玻璃瓶、30-50目筛
·去离子水、浓硫酸
试剂准备
Working Reagent I：临用前配制；每瓶加入35mL去离子水，充分溶解转移到烧杯中，缓慢加入35mL浓硫酸，充分混匀，冷却后待用，玻璃瓶避光保存。现用现配。
Reagent II：临用前配制；根据用量将浓硫酸和去离子水按照1:183的比例将浓硫酸加入去离子水中，充分混匀。（自备）
注意：Reagent I 和 Reagent II 有腐蚀性且有刺激性气味，建议在通风橱进行实验。
Standard：临用前配制；每瓶加入10mL去离子水，充分溶解，即4mg/mL有机碳标准品；用不完的试剂4°C保存2周。使用4mg/mL有机碳标准品，按照下表所示，进一步稀释标准品：
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样本制备
注意：推荐使用新鲜土壤样本。
新鲜土样自然风干或37°C烘箱风干，过30-50目筛。
实验步骤
1. 酶标仪或可见光分光光度计预热30min以上，调节波长到585nm，可见光分光光度计用去离子水调零。
2. 操作表（下述操作在消解管中操作）：
| 试剂 | 空白管 | 标准管 | 测定管 |
| 样本 (g) | 0 | 0 | 0.1 |
| Standard (mL) | 0 | 1 | 0 |
| 去离子水 (mL) | 1 | 0 | 0 |
| Working Reagent I (mL) | 2 | 2 | 2 |
| 置于消解仪上150°C消解30min，室温自然冷却。 | / | / | / |
| Reagent II (mL) | 9 | 9 | 10 |
2. SOC含量的计算
SOC (%)=x÷W÷1000×100=0.1x÷W
W：样本质量，g
1000：换算系数
1g=1000mg
结果展示
以下数据仅供参考，实验者需根据自己的实验对样品进行检测。
免责声明
本产品仅供科学研究使用，不适用于临床诊断。为了您的安全和健康，请穿实验服并戴一次性手套操作。
Soil organic carbon (SOC) refers to the carbon content in humus formed by microbial activity, plant and animal residues, and microbial biomass. According to its availability to microorganisms, soil organic carbon is classified into labile organic carbon, recalcitrant organic carbon and inert organic carbon. Labile organic carbon has high bioavailability and a high loss rate, whereas recalcitrant organic carbon has a high residual rate, generally accounting for 60%–80% of total soil organic matter, with a substantial fraction participating in humus formation. The Soil Organic Carbon (SOC) Content Assay Kit (Micro Method) is used for the detection of soil samples. In this kit, soil organic carbon in the sample is oxidized by an excess potassium dichromate-sulfuric acid solution under heating. Hexavalent chromium (Cr⁶⁺) in potassium dichromate is reduced to trivalent chromium (Cr³⁺), and the amount of Cr³⁺ produced is directly proportional to the content of organic carbon in the sample. The absorbance is measured at a wavelength of 585 nm, and the soil organic carbon content is calculated based on the concentration of trivalent chromium (Cr³⁺).
Components and Storage:
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Consumables to be Prepared Separately:
·Microplate reader or visible spectrophotometer (capable of measuring absorbance at 585 nm)
·96-well plates or micro glass cuvettes, adjustable pipettes and pipette tips, 1.5 mL centrifuge tubes
·Water bath, centrifuge, digestion instrument, digestion tubes, glass bottles, 30–50 mesh sieve
·Deionized water, concentrated sulfuric acid
Reagent Preparation:
Working Reagent I: Prepare immediately before use. Add 35 mL deionized water to each bottle to fully dissolve the contents, transfer the solution to a beaker, slowly add 35 mL concentrated sulfuric acid, mix thoroughly, cool before use, and store in a glass bottle away from light. Prepare fresh right before use.
Reagent II: Prepare immediately before use. Mix concentrated sulfuric acid and deionized water at a volume ratio of 1:183 (add concentrated sulfuric acid slowly into deionized water) and mix thoroughly. (To be prepared separately)
Note: Working Reagent I and Reagent II are corrosive and pungent. All operations should be performed in a fume hood.
Standard Solution: Prepare immediately before use. Add 10 mL deionized water to each bottle and dissolve completely to obtain a 4 mg/mL organic carbon standard stock solution. Unused stock solution can be stored at 4°C for up to 2 weeks. Further dilute the 4 mg/mL standard solution as shown below:
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Sample Preparation:
Note: Fresh soil samples are recommended.
Air-dry fresh soil samples naturally or dry them in an oven at 37°C, then sieve through a 30–50 mesh sieve.
Experimental Procedure:
1. Preheat the microplate reader or visible spectrophotometer for at least 30 minutes and set the wavelength to 585 nm. Zero the visible spectrophotometer with deionized water.
2. Operation Table (All operations are performed in digestion tubes):
| Reagent | Blank Tube | Standard Tube | Sample Tube |
| Soil sample (g) | 0 | 0 | 0.1 |
| Standard (mL) | 0 | 1 | 0 |
| Deionized water (mL) | 1 | 0 | 0 |
| Working Reagent I (mL) | 2 | 2 | 2 |
| Digest at 150°C in digestion instrument for 30 min, then cool naturally to room temperature. | / | / | / |
| Reagent II (mL) | 9 | 9 | 10 |
2. Calculation of SOC Content
SOC (%) = x ÷ W ÷ 1000 × 100 = 0.1x ÷ W
W: mass of soil sample (g)
1000: conversion factor
1g = 1000 mg
Result Presentation:
The following data is for reference only. Samples should be tested according to the actual experimental conditions.
Figure 1. Standard Curve
Figure 2. Determination of SOC content in soil samples using this assay kit
Disclaimer:
This product is for research use only and is not intended for clinical diagnosis. For your safety and health, please wear a lab coat and disposable gloves during operation.
| Item No. | 物理外观 | 组件 | 48T | 96T | 储存 |
| S1522276A | 粉末 | 试剂一 | 2 vials | 4 vials | 2-8℃. Store in the dark. |
| S1522276B | 粉末 | 标准品 | 2 vials | 2 vials | 2-8℃ |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838257 |
分析证书 | S1522276 |
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