计算溶液所需的质量、体积或浓度。
1000nt单链RNA分子量标准
有货
为什么选择此级别
BioReagent,适用于电泳 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品由体外转录得到的6条高纯度单链RNA组成，长度分别为100、200、300、400、600、1000 nt，每微升产品中RNA量约为500 ng，适用于RNA常规凝胶电泳。
产品组成
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注： 2×RNA上样缓冲液成分：95%甲酰胺，0.02% SDS，0.02%溴酚蓝，0.02%二甲苯青，1 mM EDTA。
质量控制
1. 浓度：经NanoDrop测定，本品中RNA浓度为500 ng/μl±4%。
2. 凝胶电泳：使用2% TBE凝胶电泳检测，本品的6条RNA带型清晰，无降解或弥散。
使用方法
一、普通琼脂糖凝胶电泳
1. 取1~2 μl ssRNA Marker 1000加入等体积2×RNA上样缓冲液并混匀，然后65 ℃加热10 min，迅速转移至冰上冷却3 min；样品需和ssRNA Marker进行相同的处理，可通过无核酸酶水稀释样品并补加2×RNA上样缓冲液调整体积；
2. 配制2%或更高浓度的TBE琼脂糖凝胶并电泳；
3. 将电泳完成的凝胶进行染色并拍照。
二、甲醛变性琼脂糖凝胶电泳
1. 配制3%甲醛变性琼脂糖凝胶：称取1.5 g琼脂糖加入36 ml DEPC水中，加热融化后，加入5 ml 10× MOPS Buffer，待溶液冷却至不烫手时，加入9 ml 37%甲醛溶液 (在通风橱中操作) ，轻柔混匀 (透光观察无油状物) 后倒胶，室温凝胶30~60 min；
2. ssRNA Marker 1000和样品处理方式与普通琼脂糖凝胶电泳一致；
3. 将琼脂糖凝胶放入电泳槽，在电泳槽中加入1× MOPS Buffer至没过凝胶，10 V/cm条件下预电泳10 min；
4. 上样，5~10 V/cm条件下电泳至溴酚蓝迁移至胶的2/3左右位置，电泳过程中应每隔10~20 min混匀电泳槽中的电泳液一次；
5. 电泳结束后，将凝胶在DEPC水中浸泡15 min，除去凝胶中的甲醛；
6. 染色20~30 min (避免染色过久导致RNA降解)，拍照。
结果展示
2 μl/lane, 2% TBE agarose gel
1× TBE, 6 V/cm, 65 min
注意事项
1. ssRNA Marker与普通RNA一样极易被RNase降解，因此实验时请戴好手套、口罩，实验的仪器及溶液应经DEPC处理，凝胶及电泳缓冲液应现用现配。
2. 若需得到更高质量电泳结果，可适当减小制胶厚度，满足上样量需求即可；
3. ssRNA Marker条带为单链线性RNA，适用于单链线性RNA样品的分子量参照，对于Total RNA，本产品只能作为定性参照。
4. 本产品RNA不含Poly(A)尾，无法用于逆转录实验。
5. 2×RNA上样缓冲液中含有毒害性的甲酰胺和甲醛，使用时请戴口罩、防护手套及工作服。如果使用时不慎洒到皮肤上，请用大量清水冲洗，必要时直接到医院就诊。
6. 本产品仅供科研使用。
This product consists of 6 high-purity single-stranded RNAs obtained by in vitro transcription, with lengths of 100 nt, 200 nt, 300 nt, 400 nt, 600 nt and 1000 nt respectively. The RNA content is approximately 500 ng per microliter, suitable for routine RNA gel electrophoresis.
Product Components
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Note: Composition of 2× RNA Loading Buffer: 95% Formamide, 0.02% SDS, 0.02% Bromophenol Blue, 0.02% Xylene Cyanol, 1 mM EDTA.
Quality Control
1. Concentration: Determined by NanoDrop, the RNA concentration of this product is 500 ng/μl ± 4%.
2. Gel Electrophoresis: Detected by 2% TBE gel electrophoresis, all 6 RNA bands are clear without degradation or smearing.
Instructions for Use
I. Conventional Agarose Gel Electrophoresis
1. Take 1–2 μl ssRNA Marker 1000, mix with an equal volume of 2× RNA Loading Buffer, heat at 65 ℃ for 10 min, and quickly cool on ice for 3 min. Treat RNA samples in the same manner. Samples may be diluted with nuclease-free water and adjusted to proper volume with additional 2× RNA Loading Buffer.
2. Prepare 2% or higher concentration TBE agarose gel and perform electrophoresis.
3. Stain the gel after electrophoresis and take images.
II. Formaldehyde Denaturing Agarose Gel Electrophoresis
1. Prepare 3% formaldehyde denaturing agarose gel: Weigh 1.5 g agarose and dissolve in 36 ml DEPC-treated water by heating. Add 5 ml 10× MOPS Buffer after cooling. When the solution is warm but not hot, add 9 ml 37% formaldehyde solution (operate in fume hood). Mix gently until no oily precipitate appears, pour the gel and solidify at room temperature for 30–60 min.
2. Treat ssRNA Marker 1000 and samples following the same procedure as conventional agarose gel electrophoresis.
3. Place the gel into electrophoresis tank, add 1× MOPS Buffer to submerge the gel, and pre-run electrophoresis at 10 V/cm for 10 min.
4. Load samples and run electrophoresis at 5–10 V/cm until bromophenol blue migrates to approximately two-thirds of the gel length. Mix electrophoresis buffer every 10–20 min during electrophoresis.
5. After electrophoresis, soak the gel in DEPC water for 15 min to remove residual formaldehyde.
6. Stain for 20–30 min (avoid over-staining to prevent RNA degradation) and photograph.
Result Reference
2 μl/lane, 2% TBE agarose gel
1× TBE, 6 V/cm, 65 min
Precautions
1. Like common RNA, ssRNA Marker is highly susceptible to RNase degradation. Wear gloves and masks during experiments. All instruments and reagents must be DEPC-treated. Gels and electrophoresis buffers should be freshly prepared.
2. For higher-quality electrophoresis results, appropriately reduce gel thickness while meeting sample loading requirements.
3. Bands of ssRNA Marker are linear single-stranded RNA, serving as molecular weight reference for linear ssRNA samples. It can only be used for qualitative analysis of total RNA.
4. RNA in this product has no Poly(A) tail and cannot be used for reverse transcription experiments.
5. 2× RNA Loading Buffer contains toxic formamide and formaldehyde. Wear masks, protective gloves and lab coats during operation. If spilled on skin, rinse thoroughly with plenty of water and seek medical attention if necessary.
6. For research use only.
规格
别名
RNA Marker 1000分子量标准
英文别名
RNA Ladder (100-1000nt) | RNA Marker RL1000
规格或纯度
BioReagent, 适用于电泳, 100-1000nt, 6 bands, 100nt/ 200nt/ 300nt/ 400nt/ 600nt/ 1000 nt
稳定性与储存
Store at -80℃ long term (24 months).
英文名称
ssRNA Marker 1000
储存条件
-80℃储存
运输条件
特低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0636730 |
分析证书 | S1522795 |
技术文档和文章
溶液计算器
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