计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖合酶 (Sucrose synthase, OcSUS1),E.C.:2.4.1.13,来源于 Ornithogalum caudatum。该酶可以用于合成 UDP-glucose。本产品利用大肠杆菌重组表达,纯度高,可以用于一锅多酶体系。
反应式
Neu5Ac + CTP —— CMP-Neu5Ac + PPi
使用方法
1. 按照反应体系加入底物,5mM MgCl₂,可适当加入缓冲盐 (如:Tris-HCI),调节 pH 为 7,加入适量 OcSUS1 (根据反应需求);
2. 混匀后,将样品置于 37°C 孵育 1-2h。推荐反应缓冲是 Tris-HCL (pH7),多数常见的生物缓冲也适用,比如 Tris 或 PBS。
3. 可沸水浴 5min 灭活酶,12000rpm 离心 10min,取上清检测。
【注】体系放大时,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
【注】多数常见的生物缓冲液适用,比如 Tris 或 PBS;不在此 pH 范围的缓冲液 (如醋酸缓冲液) 可能适用,但孵育时间或酶量需要根据实际情况进行优化。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
Sucrose synthase (OcSUS1), E.C 2.4.1.13, derived from Ornithogalum caudatum. This enzyme is applied for the synthesis of UDP-glucose. The product is recombinantly expressed in Escherichia coli with high purity and suitable for one-pot multi-enzyme systems.
Reaction Equation
Neu5Ac + CTP —— CMP-Neu5Ac + PPi
Usage Instructions
1.Add substrates and 5 mM MgCl₂ to the reaction system. Supplement buffer salts (e.g., Tris-HCl) as needed to adjust pH to 7, then add an appropriate amount of OcSUS1 according to reaction requirements.
2.Mix thoroughly and incubate the sample at 37 °C for 1–2 h. Tris-HCl (pH 7) is the recommended reaction buffer; most common biological buffers such as Tris and PBS are also compatible.
3.Terminate the reaction by heat inactivation in a boiling water bath for 5 min, centrifuge at 12,000 rpm for 10 min, and collect the supernatant for detection.
Notes
For scale-up reactions, incubation duration or enzyme dosage shall be adjusted based on actual conditions; gentle shaking is recommended for uniform mixing.
Most conventional biological buffers including Tris and PBS are applicable. Buffers outside this pH range (e.g., acetate buffer) may also work, yet incubation time and enzyme loading need to be optimized experimentally.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1525149 | |
| 分析证书 | S1525149 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 EnzymoPure™ 级别指南 →