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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
超氧化歧化酶（SOD）能够催化超氧阴离子（O₂・⁻）歧化，生成过生成过氧化氢 (H₂O₂) 和单质氧气(O₂)，是一种重要的抗氧化酶。目前有许多间接或者直接测定SOD的方法，其中，其中NBT (氮蓝四唑) 法由于使用方便而被广泛使用，但是，NBT法有几个缺点，例如生成的染料水溶性差，而且会和⻩嘌呤氧化酶的还原型发生反应。虽然 Cytochrome C法也常被用来做SOD检测，但它和超氧化物反应过于剧烈，不能测定低水平的SOD。SOD Assay Kit, WST-1使用了易溶于水的噻唑盐：WST-1（2-(4-碘苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯基)-2氢-四唑盐,二钠盐），能够和超氧阴离子反应生成水溶性的染料，因此可以简便地进行SOD的检测。WST-1与超氧阴离子反应的剧烈程度比 Cytochrome C小70倍；因此，它的检测灵敏度非常高，并且能够通过将样品用buffer稀释，使背景的吸光度最小化。WST-1不会和⻩嘌呤氧化酶的还原型发生反应，因此，能够检测SOD 100%的抑制率。WST-1被超氧阴离子还原的比率与⻩嘌呤氧化酶的活性线性相关，并且会被SOD所抑制（⻅下图）。因此，SOD或者SOD类似物的IC50 (50%的抑制浓度) 就能用比色法来测定。
图1. 基于⻩嘌呤氧化酶偶联反应体系和WST-1的SOD酶活力检测原理图。
WST-1的反应产物是稳定的水溶性产物，可以通过单个时间点的吸光度检测来测定SOD活力，适合高通量筛选研究。同时WST-1法测定SOD酶活力时，最大抑制百分率可以接近100%，并且可以不受一些常⻅的干扰因素的干扰，使检测效果比其它的一些常⻅方法显著改善。本试剂盒的性能优于同类产品总SOD活性检测试剂盒 (NBT法)。本产品的用途与同类产品总SOD活性检测试剂盒 (NBT法) 相同，等量SOD导致的吸光度变化值显著大于NBT法，线性范围更宽。
本试剂盒提供的SOD样品制备液能直接裂解细胞，无需匀浆，操作更加便捷。本试剂盒的检测不受样品中过氧化氢的干扰。很多细胞和组织样品中含有内源性的过氧化氢，会干扰SOD的检测。本试剂盒通过添加适量过氧化氢酶等特殊方法，能有效去除常规样品中过氧化氢的干扰。例如，对于SOD标准品的检测，标准品中添加高达0.1mM的过氧化氢时，对于检测结果仍无显著影响。本试剂盒可以检测细胞或组织匀浆液上清、全血、红细胞抽提物、血清 (浆)、胸水、腹水、肾透析液、尿液、红细胞、白细胞、血小板、心肌培养细胞、肿瘤培养细胞、各种动植物组织细胞级亚细胞等生物样品中的SOD活力。
产品组分及储存条件：
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注意事项：
1. 标准品稀释时所用的稀释液应尽量与样品一致。样品用试剂盒提供的SOD样品制备液制备时，标准品也宜使用SOD样品制备液进行稀释；当样品为血液等无需处理的样品时，宜使用试剂盒提供的SOD检测缓冲液稀释标准品。
2. 酶溶液有时会被分成两层，因此忽略移液器混合步骤会导致实验结果不准确。
3. 为提高实验准确性，建议每个样品进行复孔实验。
4. 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于⻝品或药品，不得存放于普通住宅内。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用方法：
1、样品的准备
1.1 细胞样品的准备
对于贴壁细胞，吸净细胞培养液，用4℃或冰浴预冷的PBS或生理盐水洗涤一遍，按照每100万细胞加入100-200微升的比例加入本试剂盒提供的SOD样品制备液，适当吹打以充分裂解细胞；对于悬浮细胞，600g离心5分钟收集细胞，用4℃或冰浴预冷的PBS或生理盐水洗涤一遍，按照每100万细胞加入100-200微升的比例加入SOD样品制备液，适当吹打，以充分裂解细胞。4℃约12,000g离心3-5分钟，取上清作为待测样品。
1.2 组织样品的准备
动物用生理盐水（0.9% NaCl，含有0.16mg/ml肝素钠）灌流清除血液后获取组织样品。取适量的组织样品，按照每10mg组织加入100微升SOD样品制备液的比例在4℃或冰浴进行匀浆（可以使用玻璃匀浆器或各类常见电动匀浆器）。4℃约12,000g离心3-5分钟，取上清作为待测样品。
1.3 血浆或红细胞样品的准备
用抗凝管收集血液，颠倒混匀。4℃ 600g离心10分钟，移取上清至另一新的1ml离心管中，适量生理盐水稀释后即可作为血浆样本进行检测。红细胞样品可以参考步骤1.1悬浮细胞样品的制备方法，或其它不含Triton X-100等去垢剂的样品制备方法。
注：
(1) 上述样品准备完毕后可以使用BCA蛋白浓度测定试剂盒测定蛋白浓度。推荐阿拉丁B665595 BCA蛋白定量试剂盒或R1491648 即用型BCA蛋白定量试剂盒。通常10-20微克蛋白的细胞或组织匀浆液样品中的SOD平均活力约1个活力单位左右（不同细胞和组织的差异会比较大，该活力范围仅作为初步的参考）。每种样品准备20-100微克蛋白量通常已经足够用于后续检测。
(2) 根据蛋白浓度和预计的蛋白使用量，用本试剂盒提供的SOD检测缓冲液适当稀释样品。例如，小鼠肝脏组织10%匀浆液（组织和匀浆液的重量比为1:10）上清，通常需要稀释10-100倍。准备好的样品如果当天测定，可以冰浴保存；如果当天不能完成测定，可以-70℃冻存，但建议尽量当天完成测定。
2、试剂盒的准备工作
2.1 WST-1/酶工作液的配制
按照每个反应160μl的体积配制适量的WST-1/酶工作液。均匀混合151μl SOD检测缓冲液、8μl WST-1和1μl酶溶液，即可配制成160μl WST-1/酶工作液。根据待检测样品（包括标准品）的数量，配制适量的WST-1/酶工作液。具体配制方法可以参考下表。配制好的WST-1/酶工作液4℃或冰浴保存，可以在当天使用，但建议尽量现配现用。
注：由于酶溶液的用量较少且易沉降，必须注意在使用前先轻轻离心一下，然后适当混匀后再使用。
| 待测样品数量 | 1 | 10 | 20 | 50 |
| SOD检测缓冲液 (μL) | 151 | 1510 | 3020 | 7550 |
| WST-1 (μL) | 8 | 80 | 160 | 400 |
| 酶溶液 (μL) | 1 | 10 | 20 | 50 |
| WST-1/酶工作液 (μL) | 160 | 1600 | 3200 | 8000 |
2.2 反应启动工作液的配制
把试剂盒中的反应启动液 (40×) 融解后混匀，按照每1μl反应启动液 (40×) 加入39μl SOD检测缓冲液的比例进行稀释，混匀后即为反应启动工作液。根据待检测样品（包括标准品）的数量，配制适量的反应启动工作液。配制好的反应启动工作液4℃或冰浴保存，可以在当天使用，但建议尽量现配现用。
2.3 (可选做) SOD标准品准备
需自备SOD标准品，用本试剂盒提供的SOD样品制备液（当样品用试剂盒提供的SOD样品制备液制备时）或SOD检测缓冲液（当样品为血液等无需处理的样品时）将SOD标准品稀释至如下系列浓度：100U/ml，50U/ml，20U/ml，10U/ml，5U/ml，2U/ml，1U/ml。在随后的检测中可以各取20微升，参考样品进行检测。SOD标准品的检测效果参考图2。
说明：为避免稀释后SOD酶活性的下降，SOD标准品宜现稀释现使用；本试剂盒对于SOD的检测并不需要SOD作为标准品，但可以使用SOD标准品作为阳性对照或作为对SOD活性定量的参考。
3、样品测定
3.1 参考下表使用96孔板设置样品孔和各种空白对照孔。并按下表依次加入待测样品和其它各种溶液。加入反应启动工作液后充分混匀。
注意：加入反应启动工作液后反应即会开始，可以在低温操作或用排枪操作以减小各孔间因加入反应启动工作液的时间先后差异而导致的误差。
| 样品/标准品 | 空白对照1 | 空白对照2 | 空白对照3 | |
| 待测样品 | 20μl | / | / | 20μl |
| SOD检测缓冲液 | / | 20μl | 40μl | 20μl |
| WST-1/酶工作液 | 160μl | 160μl | 160μl | 160μl |
| 反应启动工作液 | 20μl | 20μl | / | / |
注：如果样品有颜色或含有抗氧化物质，则需设置空白对照3；如果样品没有颜色并且也不含有抗氧化物则没有必要设置空白对照3。
3.2 37℃孵育30分钟。说明：孵育25至35分钟检测出来的SOD活力无显著差异，但为保证检测结果的一致性，推荐孵育30分钟。
3.3 在450nm测定吸光度。如无450nm滤光片，可以使用420-480nm的滤光片。可以选择设定600nm（或600nm以上，如650nm）作为参比波长（也称参考波长），450nm吸光度的读数扣除参比波长的吸光度读数即可作为实测读数。
4、样品中总SOD活力的计算
4.1 抑制百分率的计算
参考如下计算公式计算抑制百分率：
抑制百分率＝[(A空白对照1－A空白对照2)－(A样品－A空白对照3)] / (A空白对照1－A空白对照2)×100%
如果样品没有颜色并且也不含有抗氧化物，则A空白对照2=A空白对照3，此时可以把计算公式简化为如下形式（简化时可以不设置空白对照3）：
抑制百分率＝(A空白对照1－A样品) / (A空白对照1－A空白对照2) × 100%
注：如果计算出来的抑制百分率小于30%或大于70%，则通常需要把该样品重新测定。尽量使样品的抑制百分率在30-70%范围内。如果测定出来的抑制百分率偏高，则需适当稀释样品；如果测定出来的抑制百分率偏低，则需重新准备浓度较高的待测样品。
4.2 SOD酶活力单位的定义
在上述黄嘌呤氧化酶偶联反应体系中抑制百分率为50%时，反应体系中的SOD酶活力定义为一个酶活力单位（unit）。注意：SOD的活力单位的定义方式有很多种，不同的活力单位需根据其定义的不同进行适当换算。
4.3 SOD酶活力的计算
图2. 本试剂盒对SOD标准品的检测效果
注：图A纵坐标ΔA450为孵育30分钟后，空白对照1与SOD标准品孔的吸光度差值。
SOD酶活力和ΔA450及抑制百分率 (图B) 呈非线性关系，而1/SOD酶活力和1/抑制百分率成线性关系 (图C)。
图中数据仅作参考，实际测定获得的标准曲线的斜率和截距可能会因具体反应条件的不同和上图有较明显的差别。
SOD酶活力的计算公式如下
待测样品中SOD酶活力单位＝检测体系中SOD酶活力单位＝抑制百分率 / (1－抑制百分率) units
例如，当抑制百分率为50%时，待测样品中SOD酶活力单位＝50% / (1－50%) units＝1 unit；当抑制百分率为60%时，待测样品中SOD酶活力单位＝60% / (1－60%) units＝1.5 units。
4.4 如果样品为细胞或组织的匀浆液，可以根据样品的蛋白浓度和稀释倍数，将SOD活力单位换算为U/g或U/mg蛋白。如果样品为红细胞抽提液，可以根据血红蛋白含量，可换算为U/克血红蛋白或U/毫克血红蛋白。
附1：SOD酶活力计算的参考方案：可以先使用本试剂盒绘制SOD标准品的抑制百分率曲线，然后根据样品检测到的抑制百分率对比标准品的抑制百分率曲线计算出样品中的SOD酶活力单位。本方案仅供参考，使用本试剂盒时不必使用本方案进行检测和计算。此外，本方案需确保标准品的酶活力数据可靠，不会因为标准品的保存问题而导致实际酶活力下降。
附2：SOD酶活力的动力学检测：如果条件许可，使用本试剂盒时也可以使用动力学方法检测SOD的酶活力。通常在步骤3.1后可以37℃孵育同时在450nm连续测定吸光度30分钟。根据30分钟内的吸光度变化的斜率计算出抑制百分率：
抑制百分率＝[(斜率空白对照1－斜率空白对照2)－(斜率样品－斜率空白对照3)]/(斜率空白对照1－斜率空白对照2)×100%
其余的计算方法同上述非动力学的计算方法。动力学方法的检测和计算更加精确一些，但检测起来相对要麻烦一些。使用本试剂盒通常使用非动力学方法即可。
注意事项
本产品仅供科学研究使用，不适用于临床诊断。
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide anions (O₂•⁻) to yield hydrogen peroxide (H₂O₂) and oxygen gas (O₂), and it is an essential antioxidant enzyme. Numerous direct and indirect methods are currently available for quantifying SOD activity. Among these methods, the NBT (nitro blue tetrazolium) assay is widely adopted for its ease of operation, yet it carries several drawbacks. For instance, the dye product formed exhibits poor water solubility and can react with the reduced form of xanthine oxidase. The Cytochrome C assay is another common approach for SOD measurement; however, its reaction with superoxide proceeds too rapidly, rendering it unsuitable for detecting low SOD concentrations.
The WST-1 SOD Assay Kit employs a water-soluble thiazolium salt, WST-1 (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, disodium salt). This reagent reacts with superoxide anions to generate a water-soluble chromogenic product, enabling simple and convenient SOD quantification. The reaction rate of WST-1 with superoxide anions is 70 times slower than that of Cytochrome C. Accordingly, the kit delivers superior detection sensitivity, and sample dilution with assay buffer minimizes background absorbance. Since WST-1 does not interact with the reduced form of xanthine oxidase, it can measure the full 100% inhibition rate of SOD. The rate of WST-1 reduction by superoxide anions correlates linearly with xanthine oxidase activity, and this reduction reaction is inhibited by SOD (see figure below). As such, the IC₅₀ (half-maximal inhibitory concentration) of SOD or SOD mimetics can be determined via colorimetric spectrophotometry.
Figure 1. Schematic diagram of SOD activity assay based on xanthine oxidase coupled reaction system and WST-1.
The reaction product of WST-1 is a stable water-soluble chromogen. SOD activity can be quantified by measuring absorbance at a single time point, making this method suitable for high-throughput screening studies. Meanwhile, the WST-1 method achieves a maximum inhibition percentage close to 100% when measuring SOD activity, and it is resistant to interference from many common confounding factors, delivering significantly improved detection performance compared with other conventional assays. This kit outperforms the comparable Total SOD Activity Detection Kit (NBT method). It shares the same application scope as the NBT-based total SOD kit; under equivalent SOD loading, the absorbance variation measured with this kit is markedly higher, accompanied by a broader linear detection range.
The SOD Sample Preparation Solution provided in this kit can directly lyse cells without homogenization, greatly simplifying experimental operation. The assay is not interfered by hydrogen peroxide present in samples. Endogenous hydrogen peroxide widely exists in cell and tissue specimens and will disturb SOD measurement. Our kit incorporates catalase and other proprietary components to effectively eliminate hydrogen peroxide interference in routine samples. For instance, adding hydrogen peroxide up to 0.1 mM to SOD standard solutions exerts no significant impact on detection results. This kit enables quantification of SOD activity in various biological specimens, including supernatants of cell or tissue homogenates, whole blood, red blood cell extracts, serum/plasma, pleural effusion, ascites, renal dialysate, urine, red blood cells, white blood cells, platelets, cultured cardiomyocytes, cultured tumor cells, and diverse animal/plant tissue cells and subcellular fractions.
Product Components and Storage Conditions:
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Precautions:
1. The diluent used for standard dilution should be consistent with that for samples as much as possible. If samples are prepared with the SOD Sample Preparation Solution supplied in the kit, the standard should also be diluted with the same solution. For untreated samples such as blood, dilute the standard with the provided SOD Assay Buffer.
2. The Enzyme Solution may separate into two layers. Skipping pipette mixing will lead to inaccurate experimental results.
3. It is recommended to set replicate wells for each sample to improve experimental accuracy.
4. This product is for scientific research use only by professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.
5. Wear a lab coat and disposable gloves during operation for your personal safety and health.
Experimental Procedure
1. Sample Preparation
1.1 Cell Sample Preparation
Adherent Cells: Aspirate the culture medium. Wash cells once with ice-cold PBS or physiological saline. Add 100-200 µL of the provided SOD Sample Preparation Solution per 1 million cells. Pipette gently to lyse cells thoroughly.
Suspension Cells: Collect cells by centrifugation at 600 g for 5 min. Wash the cell pellet once with ice-cold PBS or physiological saline. Add 100-200 µL of SOD Sample Preparation Solution per 1 million cells. Pipette gently to lyse cells thoroughly.
Centrifuge all lysates at 12,000 g for 3-5 minutes at 4°C. Collect the supernatant as the test sample.
1.2 Tissue Sample Preparation
Perfuse animals with physiological saline (0.9% NaCl containing 0.16 mg/ml heparin sodium) to remove blood before collecting tissue samples.
Take an appropriate amount of tissue and homogenize it on ice using 100 µL of SOD Sample Preparation Solution per 10 mg tissue.
Centrifuge the homogenate at 12,000 g for 3-5 minutes at 4°C. Collect the supernatant as the test sample.
1.3 Plasma or Red Blood Cell Sample Preparation
Collect blood in an anticoagulant tube and mix gently by inverting.
Centrifuge at 600 g for 10 min at 4°C. Transfer the supernatant (plasma) to a new tube. Dilute appropriately with physiological saline before assay.
For red blood cell samples, refer to the method for suspension cells in section 1.1, or other sample preparation methods that do not contain detergents such as Triton X-100.
Notes: After preparation, determine the protein concentration using a BCA Protein Assay Kit. Typically, 10-20 µg of protein from cell or tissue homogenates contains approximately 1 unit of SOD activity (varies significantly). Preparing 20-100 µg of protein is usually sufficient.
Dilute the sample appropriately with the provided SOD Assay Buffer based on the protein concentration and intended protein amount. For example, a 10% mouse liver homogenate supernatant usually requires a 10-100-fold dilution. Keep prepared samples on ice if assaying the same day; store at -70°C if not, but same-day assay is recommended.
2. Kit Reagent Preparation
2.1 WST-1/Enzyme Working Solution Preparation
Prepare sufficient WST-1/Enzyme Working Solution for the number of assays (including standards), using 160 µL per reaction.
For each 160 µL, mix 151 µL SOD Assay Buffer, 8 µL WST-1, and 1 µL Enzyme Solution uniformly.
Refer to the table below for bulk preparation. Prepare fresh before use; keep on ice if stored temporarily during the day.
| Number of Tests | 1 | 10 | 20 | 50 |
| SOD Assay Buffer (µL) | 151 | 1510 | 3020 | 7550 |
| WST-1 (μL) | 8 | 80 | 160 | 400 |
| Enzyme Solution (μL) | 1 | 10 | 20 | 50 |
| Total Volume (µL) | 160 | 1600 | 3200 | 8000 |
Note: Centrifuge the Enzyme Solution vial briefly before use and mix gently due to its small volume and tendency to settle.
2.2 Reaction Initiation Working Solution Preparation
Thaw and mix the Reaction Initiation Solution (40×). Dilute it 1:39 with SOD Assay Buffer (e.g., 1 µL reagent + 39 µL Buffer). Mix well.
Prepare sufficient volume for all assays (including standards). Prepare fresh before use; keep on ice if stored temporarily during the day.
2.3 (Optional) SOD Standard Preparation
Prepare an SOD standard yourself. Dilute it serially to the following concentrations using the SOD Sample Preparation Solution (if samples were prepared with it) or SOD Assay Buffer (for direct samples like blood): 100, 50, 20, 10, 5, 2, 1 U/mL.
Use 20 µL per well in the assay as a reference. See Figure 2 for expected results.
Note: Dilute the SOD standard immediately before use to prevent activity loss. The kit does not require an SOD standard for detection but can use it as a positive control or for quantitative reference.
3. Sample Assay
3.1 Plate Setup
Set up sample wells and various blank control wells in a 96-well plate according to the table below.
Add reagents in the specified order. Mix thoroughly after adding the Reaction Initiation Working Solution.
Note: The reaction starts immediately after adding the Reaction Initiation Working Solution. Operate at low temperatures or use a multi-channel pipette to minimize timing errors between wells.
| Reagent | Sample / Standard | Blank 1 | Blank 2 | Blank 3 |
| Sample | 20μl | / | / | 20μl |
| SOD Assay Buffer | / | 20μl | 40μl | 20μl |
| WST-1/Enzyme Working Solution | 160μl | 160μl | 160μl | 160μl |
| Reaction Initiation Working Solution | 20μl | 20μl | / | / |
Note: Set up Blank 3 if the sample is colored or contains antioxidants. If not, Blank 3 is unnecessary.
3.2 Incubation
Incubate at 37°C for 30 minutes. Incubation times between 25-35 minutes show no significant difference, but 30 minutes is recommended for consistency.
3.3 Absorbance Measurement
Measure the absorbance at 450 nm. A filter in the range of 420-480 nm can be used.
It is recommended to use a reference wavelength (e.g., 600 nm or 650 nm). Subtract the reference wavelength absorbance reading from the 450 nm reading for the final measured value (ΔA).
4. Calculation of Total SOD Activity
4.1 Calculation of Inhibition Percentage
Calculate the inhibition percentage using the following formula:
Inhibition Percentage (%) = [(ABlank1 - ABlank2) - (ASample - ABlank3)] / (ABlank1 - ABlank2) × 100%
If the sample is colorless and contains no antioxidants, A<sub>Blank2</sub> ≈ A<sub>Blank3</sub>, and the formula simplifies to (Blank 3 can be omitted):
Inhibition Percentage (%) = (ABlank1 - ASample) / (ABlank1 - ABlank2) × 100%
Note: If the calculated inhibition percentage is <30% or >70%, re-assay the sample after appropriate dilution (if % is high) or concentration (if % is low). Aim for 30-70% inhibition for accurate results.
4.2 Definition of SOD Enzyme Activity Unit
One unit (U) of SOD activity is defined as the amount of enzyme that causes 50% inhibition in the described xanthine oxidase-coupled reaction system.
Note: SOD activity units can be defined in various ways; different units require appropriate conversion based on their definitions.
4.3 Calculation of SOD Enzyme Activity
Figure 2. Detection performance of this kit for SOD standards.
Figure A: Y-axis ΔA<sub>450</sub> is the absorbance difference between Blank 1 and the SOD standard well after 30 min incubation.
Figure B: SOD enzyme activity has a nonlinear relationship with both ΔA<sub>450</sub> and Inhibition Percentage.
Figure C: 1/SOD enzyme activity has a linear relationship with 1/Inhibition Percentage.
Note: Data are for reference only; actual standard curve parameters may vary.
The SOD enzyme activity in the test sample can be calculated using the following formula:
SOD Activity in Test Sample (Units) = Inhibition Percentage / (1 - Inhibition Percentage)
Examples:
50% Inhibition: Activity = 50% / (1 - 50%) = 1 Unit
60% Inhibition: Activity = 60% / (1 - 60%) = 1.5 Units
4.4 Normalization
For cell or tissue homogenates, normalize the SOD activity units to U/mg protein or U/g tissue based on the sample's protein concentration and dilution factor.
For red blood cell extracts, normalize to U/g hemoglobin or U/mg hemoglobin based on the hemoglobin content.
Appendix 1: Alternative Calculation Method (Reference)
Plot an inhibition percentage curve using the SOD standard. Calculate the sample SOD activity by comparing the sample's inhibition percentage to this curve. This method requires a reliable SOD standard and is not mandatory for using this kit.
Appendix 2: Kinetic Assay of SOD Activity
For increased precision, perform a kinetic read: after step 3.1, incubate at 37°C and continuously measure the absorbance at 450 nm for 30 minutes. Calculate the inhibition percentage using the slopes (rates) of absorbance change:
Inhibition Percentage (%) = [(SlopeBlank1 - SlopeBlank2) - (SlopeSample - SlopeBlank3)] / (SlopeBlank1 - SlopeBlank2) × 100%
Subsequent calculations remain the same. The kinetic method is more precise but more complex.
Precautions
This product is for research use only. Not for use in diagnostic procedures.
| S1525391 | 物理外观 | 组件 | 100T | 储存 |
| S1525391A | 液体 | SOD样品制备液 | 50 ml | -20℃. Store in the dark. |
| S1525391B | 液体 | SOD检测缓冲液 | 50 ml | -20℃. Store in the dark. |
| S1525391C | 液体 | WST-1 | 0.8 ml | -20℃. Store in the dark. |
| S1525391D | 液体 | 酶溶液 | 0.1 ml | -20℃. Store in the dark. |
| S1525391E | 液体 | 反应启动液 (40×) | 0.06 ml | -20℃. Store in the dark. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0938969 |
分析证书 | S1525391 |
危险品化学品经营许可证（带存储）