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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
超氧阴离子自由基是生物体代谢过程中产生的一种活性氧，可攻击脂质、蛋白质、核酸及多不饱和脂肪酸等生物大分子，使其交联或断裂，导致细胞结构和功能损伤，与机体衰老及多种疾病密切相关，因此清除超氧阴离子自由基的研究已受到广泛关注。 本试剂盒的测定原理为：AP-TEMED系统产生超氧阴离子，与盐酸羟胺反应生成NO₂⁻，NO₂⁻再与对氨基苯磺酸和α-萘胺作用生成红色偶氮化合物，该产物在530 nm处具有特征吸收峰；样品对超氧阴离子的清除能力与530 nm处吸光值呈负相关，通过测定吸光度即可评估样品的抗氧化活性。
Superoxide anion radical is a type of free radical generated during normal metabolic processes in living organisms. It can attack biological macromolecules such as lipids, proteins, nucleic acids, and polyunsaturated fatty acids, causing cross-linking or fragmentation, leading to cellular structural and functional damage, which is closely associated with aging and various pathological conditions. Therefore, research on the scavenging of superoxide anion radicals has received widespread attention. The principle of this kit is as follows: The AP-TEMED system generates superoxide anion, which reacts with hydroxylamine hydrochloride to produce NO₂⁻. The NO₂⁻ then reacts with p-aminobenzenesulfonic acid and α-naphthylamine to form a red azo compound, which exhibits a characteristic absorbance peak at 530 nm. The scavenging capacity of the sample against superoxide anion is inversely correlated with the absorbance at 530 nm. The antioxidant activity of the sample can be evaluated by measuring the absorbance.
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