BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
可溶性淀粉合成酶（SSS，EC 2.4.1.21）以游离态存在于质体基质中，催化淀粉链的延长反应，主要负责支链淀粉的合成。SSS催化ADPG与淀粉引物（葡聚糖）反应，将葡萄糖基转移至引物上，同时生成ADP。反应体系中偶联的酶促混合物可依次将NADP⁺还原为NADPH，且NADPH的生成量与ADP的生成量呈正比。 传统方法通过检测340 nm处NADPH的增加量来测定SSS活性，但该法灵敏度较低，且易受样品中色素（如绿色叶片）干扰。本试剂盒提供一种简单、灵敏、快速的替代方案：酶促反应生成的NADPH与特异性显色探针反应形成有色产物，通过测定450 nm处该产物的生成速率，即可计算SSS活性。
Soluble starch synthase (SSS, EC 2.4.1.21) exists in a free form in the stroma of plastids and catalyzes the elongation of starch chains, being primarily responsible for the synthesis of amylopectin. SSS catalyzes the reaction of ADPG with a starch primer (glucan), transferring a glucosyl moiety to the primer and simultaneously generating ADP. A coupled enzymatic mixture in the reaction system sequentially reduces NADP⁺ to NADPH, and the amount of NADPH produced is directly proportional to the amount of ADP generated. Traditional methods determine SSS activity by measuring the increase in NADPH at 340 nm. However, these methods suffer from relatively low sensitivity and are prone to interference from pigments (e.g., green leaves) present in the samples. This kit provides a simple, sensitive, and rapid alternative: the NADPH generated from the enzymatic reaction reacts with a specific chromogenic probe to form a colored product. By measuring the rate of formation of this product at 450 nm, the SSS activity can be calculated.
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