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6-磷酸山梨醇脱氢酶（S6PDH，EC 1.1.1.200），又称醛糖6-磷酸还原酶（A6PR），广泛分布于苹果叶片等蔷薇科植物中，催化D-山梨糖醇6-磷酸与D-葡萄糖6-磷酸之间的相互转化，在山梨醇的合成代谢中发挥关键作用。该酶在正向反应中催化D-葡萄糖6-磷酸还原为D-山梨糖醇6-磷酸，同时将NADPH氧化为NADP⁺。基于上述反应，本试剂盒采用连续监测法测定S6PDH活性：在340 nm处实时追踪NADPH特征吸收峰的下降速率，在底物饱和条件下，单位时间内OD₃₄₀的初始线性降幅（ΔOD₃₄₀/min）与样本中S6PDH酶活性呈正比，据此即可精准计算酶活力大小。 反应方程式：D-葡萄糖6-磷酸 + NADPH + H⁺ → D-山梨糖醇6-磷酸 + NADP⁺
6‑Phosphosorbitol dehydrogenase (S6PDH, EC 1.1.1.200), also known as aldose 6‑phosphate reductase (A6PR), is widely distributed in Rosaceae plants such as apple leaves. It catalyzes the reversible interconversion between D‑sorbitol 6‑phosphate and D‑glucose 6‑phosphate, playing a key role in the anabolism of sorbitol. In the forward reaction, this enzyme catalyzes the reduction of D‑glucose 6‑phosphate to D‑sorbitol 6‑phosphate, concomitantly oxidizing NADPH to NADP⁺. Based on this reaction, this kit employs a continuous monitoring method for the determination of S6PDH activity: the rate of decrease in the characteristic absorption peak of NADPH at 340 nm is monitored in real time. Under substrate‑saturating conditions, the initial linear decrease in absorbance per unit time (ΔOD₃₄₀/min) is directly proportional to the S6PDH activity in the sample, allowing for accurate calculation of the enzyme activity. Reaction equation: D‑Glucose 6‑phosphate + NADPH + H⁺ → D‑Sorbitol 6‑phosphate + NADP⁺
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