BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
山梨醇是蔷薇科植物（如苹果、梨等）中主要的运输糖，其被转运到果实后转化为其他糖类物质。山梨醇氧化酶（SOX）是这一转化过程中的关键酶之一，催化山梨醇氧化为葡萄糖，与果实品质形成及糖类积累密切相关。 本试剂盒采用DNS比色法测定SOX活力。其原理为：SOX催化山梨醇生成葡萄糖；葡萄糖在碱性条件下与3,5-二硝基水杨酸（DNS）发生氧化还原反应，生成棕红色的3-氨基-5-硝基水杨酸，该产物在540 nm处具有特征吸收峰。在既定线性范围内，540 nm处吸光度的上升速率与样品中SOX活力呈正比，通过标准曲线即可定量计算酶活性。
Sorbitol is the primary translocated sugar in Rosaceae plants such as apple and pear. After being transported to the fruit, it is converted into other sugars. Sorbitol oxidase (SOX) is one of the key enzymes involved in this conversion process, catalyzing the oxidation of sorbitol to glucose, and is closely associated with fruit quality formation and sugar accumulation. This kit employs the DNS colorimetric method for the determination of SOX activity. The principle is as follows: SOX catalyzes the conversion of sorbitol to glucose. Under alkaline conditions, glucose undergoes an oxidation‑reduction reaction with 3,5‑dinitrosalicylic acid (DNS) to generate brown‑red 3‑amino‑5‑nitrosalicylic acid, which exhibits a characteristic absorption peak at 540 nm. Within the established linear range, the rate of increase in absorbance at 540 nm is directly proportional to the SOX activity in the sample, and the enzyme activity can be quantitatively calculated using a standard curve.
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