BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
三磷酸腺苷（ATP）是生物体内能量转换的最基本载体和最直接的能量来源，测定ATP含量并计算能荷，可有效反映机体的能量代谢状态。本试剂盒采用肌酸激酶偶联磷钼酸比色法测定ATP含量：在肌酸激酶（CK）催化下，ATP与肌酸反应生成磷酸肌酸和ADP；磷酸肌酸进一步水解为无机磷（Pi），无机磷在酸性条件下与钼酸铵反应生成磷钼酸复合物，该复合物被还原剂（如硫酸亚铁或抗坏血酸）还原生成蓝色的磷钼蓝（Molybdenum Blue）产物，该产物在700 nm处具有特征吸收峰。通过监测700 nm处吸光值的增幅，在底物饱和条件下，单位时间内OD₇₀₀的初始线性增幅（ΔOD₇₀₀/min）与ATP含量呈正比，据此即可精准计算样品中的ATP浓度。
Adenosine triphosphate (ATP) is the most fundamental carrier and the most direct source of energy in biological energy conversion. The determination of ATP content and the calculation of the energy charge can effectively reflect the energy metabolism status of an organism. This kit employs a creatine kinase‑coupled phosphomolybdic acid colorimetric method for the determination of ATP content. The principle is as follows: Under the catalysis of creatine kinase (CK), ATP reacts with creatine to generate phosphocreatine and ADP. Phosphocreatine is further hydrolyzed to inorganic phosphate (Pi). Under acidic conditions, Pi reacts with ammonium molybdate to form a phosphomolybdic acid complex, which is then reduced by a reducing agent (such as ferrous sulfate or ascorbic acid) to generate a blue molybdenum blue product. This product exhibits a characteristic absorption peak at 700 nm. By monitoring the increase in absorbance at 700 nm under substrate‑saturating conditions, the initial linear increase in absorbance per unit time (ΔOD₇₀₀/min) is directly proportional to the ATP content, allowing for accurate calculation of the ATP concentration in the sample.
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