This kit utilizes soil β‑1,4‑glucanase (cellobiosidase) to catalyze the hydrolysis of the substrate p‑nitrophenyl cellobioside, generating p‑nitrophenol. The product exhibits a characteristic absorption peak at 400 nm. By measuring the change in absorbance at this wavelength, the activity of soil β‑1,4‑glucanase/cellobiosidase can be quantitatively characterized.