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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤β-半乳糖苷酶(β-GAL,EC 3.2.1.23),又称β-D-半乳糖苷半乳糖基转移酶,参与土壤中碳水化合物的水解过程。本试剂盒提供一种简单、灵敏、快速的测定方法:β-GAL催化底物对-硝基苯-β-D-吡喃半乳糖苷水解,生成对-硝基苯酚(PNP),该产物在405 nm处具有特征吸收峰。通过动力学模式连续监测单位时间内OD₄₀₅的初始线性增幅(ΔOD₄₀₅/min),即可精准计算土壤样品中β-GAL的酶活力大小。
Soil β-galactosidase (β-GAL, EC 3.2.1.23), also known as β-D-galactoside galactohydrolase or β-D-galactosidase, participates in the hydrolysis of carbohydrates in soil. This kit provides a simple, sensitive, and rapid assay method: β-GAL catalyzes the hydrolysis of the substrate p‑nitrophenyl‑β‑D‑galactopyranoside to generate p‑nitrophenol (PNP), which exhibits a characteristic absorption peak at 405 nm. By continuously monitoring the initial linear increase in OD₄₀₅ per unit time (ΔOD₄₀₅/min) in kinetic mode, the enzyme activity of β‑GAL in soil samples can be accurately calculated.