Name: 土壤铵态氮含量检测试剂盒 (靛酚蓝, 比色法)
SKU: S1527372
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

🧪

为什么选择此级别

BioReagent,标曲法级，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

2-8°C储存,避光。低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

土壤中的铵态氮可被土壤胶体吸附，呈现交换性铵状态氮肥，也可溶解在土壤溶液中，直接被植物吸收利用，属于速效性氮素。铵态氮指标对农业生产具有极其重要的作用。

测定原理：

土壤中的铵态氮在强碱性介质中与次氯酸盐和苯酚作用，生成水溶性染料靛酚蓝，在 625nm 处有特征吸收峰，吸光值与铵态氮含量成正比。

注：正式测定前务必取2-3个预期差异较大的样本做预测定

组分与储存：

组分货号	组分名称	物理外观	50T	储存
S1527372A	提取液	液体	55 mL	2-8℃.
S1527372B	试剂一	粉末	2 vial	2-8°C. 避光
S1527372C	试剂二	液体	20 mL	2-8°C. 避光
S1527372D	试剂三	液体	5 mL	2-8℃.
S1527372E	标准品	液体	1 vial	2-8℃.

试剂一：临用前根据用量每瓶加 10mL 双蒸水溶解，现配现用，避光 4℃可保存 2 周。
标准品 (用于制作标准曲线)：100μg/mL NH₄⁺‑N 标准液。

自备仪器和试剂：

⁕ 可见分光光度计 (能测625nm处的吸光度)

⁕ 电子天平、台式离心机、可调式移液器、1mL 玻璃比色皿、振荡仪、恒温水浴锅

⁕ 双蒸水

样本处理：

按照土壤质量 (g)：提取液体积 (mL) 为 1：5~10 的比例 (建议称取约 0.1g 新鲜土样，加入 1mL 提取液)，振荡提取 1h，10000g ，25℃离心 10min，取上清液待测。

测定步骤：

分光光度计预热 30min，625nm 处蒸馏水调零。

试剂名称 (µL)	空白管	测定管
样本	/	100
提取液	100	/
试剂一	400	400
试剂二	400	400
/	充分混匀，25℃静置 1h	充分混匀，25℃静置 1h
试剂三	100	100

充分混匀，625nm 处测定吸光值，记为A_空白和A_测定，ΔA=A_测定-A_空白。
注意：空白管仅需测定 1-2 次。

铵态氮含量计算公式：

标准条件下建立的回归方程为 y=0.07658x+0.01681，R² =0.9985，其中 y 为ΔA，x 为铵态氮浓度 (μg/mL)。

浓度 (μg/mL)

NH₄⁺‑N 含量 (μg/g 鲜重)= (ΔA-0.01681)÷0.07658÷W=13.058×(ΔA-0.01681)÷W

W：样本质量，g（为 1mL 提取液中加入的新鲜土样的质量）

注意事项：

1. ΔA 线性范围为 0.02-1.9，产物生成浓度检测范围为 0.2-25μg/mL；如果ΔA 大于 1.9，建议用提取液稀释待测液后重新测定 (计算公式中乘以相应稀释倍数)，如果ΔA 小于 0.02，建议适当增加样本量。

2. 土壤经风干或者烘干很容易引起铵态氮含量的变化，建议采用新鲜土壤进行测定。样品采集后应于 4℃下密封保存，并在 3 天内分析完毕。否则应于-20℃ (深度冷冻) 下以小份保存，样品中氨氮可以保存数周。当测定深度冷冻氨氮含量时，应控制解冻的温度和时间。室温环境下解冻时，需在 4h 内完成。

3. 试剂一配制后应尽快使用，4℃保存下两周内有效，出现变色情况应立即停止使用。

实验实例：

取新鲜土壤，按样本处理步骤操作并进行测定，测定结果如下：

新鲜土壤样本	样 1	样 2	样 3
质量 (g)	0.1208	0.1220	0.1032
A_测定	0.136	0.114	0.128
A_空白	0.044	0.044	0.044
ΔA	0.092	0.07	0.084
NH₄⁺‑N 含量 (μg/g 鲜重)	8.128	5.693	8.502

常见问题及解决方案：

问题	可能原因	解决方法
样品读数重复性差	样品类型不合适	参考说明书了解不兼容的样品类型。
	样品提取不充分	延长振荡时间。
	样品反复冻融次数过多	将样品分装，不可反复冻融。
	样品中含有干扰物质	排查干扰物质，必要时对样品进行脱蛋白处理。
	样品读数超出线性范围	对样品进行浓缩或稀释，使读数处于线性范围内。
	孔中有气泡	气泡会干扰读数，操作时尽量避免产生气泡，读数前务必去除气泡。
样品和标准品读数偏低/偏高	样品放置时间过长或储存不当	使用新鲜制备的样品，按推荐温度保存至使用前。
	试剂盒组分储存不当	按说明书要求进行储存。
	提取时间/温度不当	参考说明书推荐的提取时间和/或温度。
	试剂加样量错误	检查移液枪是否校准 (始终使用能吸取整体积的最小量程移液枪)；尽量避免吸取过小体积的试剂。
	配制反应混合液时移液误差	始终配制混合母液。

Ammonium‑nitrogen in soil can be adsorbed by soil colloids to form exchangeable‑ammonium nitrogen fertilizer. It can also dissolve in the soil solution and be directly absorbed and utilized by plants, belonging to readily‑available nitrogen. The indicator of ammonium‑nitrogen is extremely important for agricultural production.

Determination principle:

In a strongly alkaline medium, ammonium‑nitrogen in soil reacts with hypochlorite and phenol to produce the water‑soluble dye indophenol blue, which has a characteristic absorption peak at 625 nm. The absorbance value is positively proportional to the content of ammonium‑nitrogen.

Note: It is imperative to perform a pilot assay on 2–3 samples expected to show significant differences prior to the formal measurement.

Contents & Storage:

Component No.	Component Name	Appearance	50T	Storage
S1527372A	Extraction Buffer	Liquid	55 mL	2-8℃.
S1527372B	Reagent 1	Powder	2 vial	2-8°C. Store in the dark.
S1527372C	Reagent 2	Liquid	20 mL	2-8°C. Store in the dark.
S1527372D	Reagent 3	Liquid	5 mL	2-8℃.
S1527372E	Standard	Liquid	1 vial	2-8℃.

Reagent 1: Dissolve each bottle with 10 mL double‑distilled water according to the required amount before use. Prepare freshly before use. The prepared solution can be stored at 4 °C in the dark for 2 weeks.
Standard (for standard‑curve preparation): 100 μg/mL NH₄⁺‑N standard solution.

Instruments and Reagents to Be Prepared by User:

⁕ Visible‑spectrophotometer (capable of measuring absorbance at 625 nm)

⁕ Electronic balance, bench‑top centrifuge, adjustable‑volume pipettes, 1 mL glass cuvettes, shaker, constant‑temperature water‑bath

⁕ ddH₂O

Sample Treatment:

Mix fresh soil sample with Extraction buffer at a soil mass (g)‑to‑Extraction buffer volume (mL) ratio of 1:5‑10. It is recommended to weigh approximately 0.1 g fresh soil sample and add 1 mL Extraction buffer. Perform shake extraction for 1 h, then centrifuge at 10 000 g and 25 °C for 10 min. Collect the supernatant for testing.

Measurement Procedure:

Warm up the spectrophotometer for 30 min, and zero the instrument with distilled water at 625 nm.

Reagent (µL)	Blank tube	Assay tube
Sample	/	100
Extraction buffer	100	/
Reagent 1	400	400
Reagent 2	400	400
/	Mix thoroughly and incubate at 25 °C for 1 h	Mix thoroughly and incubate at 25 °C for 1 h
Reagent 3	100	100

Mix thoroughly, then measure the absorbance at 625 nm. Record the absorbance values as A_blank and A_assay. ΔA = A_assay − A_blank.
Note: The blank tube only needs to be measured 1‑2 times.

Calculation formula for ammonium‑nitrogen content:

The regression equation established under standard conditions is y = 0.07658x + 0.01681, R² = 0.9985. y represents ΔA, and x is the concentration of ammonium‑nitrogen (μg/mL).

Concentration (μg/mL)

NH₄⁺‑N content (μg/g fresh weight) = (ΔA − 0.01681) ÷ 0.07658 ÷ W = 13.058 × (ΔA − 0.01681) ÷ W

W: mass of sample, g (the weight of fresh soil sample added into 1 mL Extraction buffer)

Precautions:

1. The linear range of ΔA is 0.02‑1.9, and the detectable concentration range of the formed product is 0.2‑25 μg/mL. If ΔA is higher than 1.9, dilute the test sample with Extraction buffer before re‑measurement (multiply the final result by the corresponding dilution factor in the calculation formula). If ΔA is lower than 0.02, appropriately increase the sample mass.

2. Air‑drying or oven‑drying of soil may easily change ammonium‑nitrogen content. Fresh soil is recommended for determination. After sampling, seal and store samples at 4 °C, and complete the analysis within 3 days. Otherwise, store samples in small aliquots at −20 °C (deep‑freezing), under which ammonium‑nitrogen can be preserved for several weeks. When measuring ammonium‑nitrogen in deep‑frozen samples, thawing temperature and duration should be well‑controlled. If thawed at room temperature, the assay must be finished within 4 h.

3. Reagent 1 should be used as soon as possible after preparation. It is valid for 2 weeks when stored at 4 °C. Discontinue use immediately if color change occurs.

Experimental example:

Fresh soil samples were treated and measured according to the sample‑treatment procedure. The results are shown below:

Fresh Soil Sample	Sample 1	Sample 2	Sample 3
Mass (g)	0.1208	0.1220	0.1032
A_assay	0.136	0.114	0.128
A_blank	0.044	0.044	0.044
ΔA	0.092	0.07	0.084
NH₄⁺‑N Content (μg/g fresh weight)	8.128	5.693	8.502

Frequently Asked Questions and Solutions:

Problem	Possible Cause	Solution
Poor reproducibility of sample readings	Inappropriate sample type.	Refer to the instruction manual for incompatible sample types.
	Insufficient sample extraction.	Prolong the shaking‑extraction time.
	Excessive freeze-thaw cycles of samples.	Aliquot samples to avoid repeated freeze-thaw cycles.
	Interfering substances present in samples.	Identify interfering substances and perform deproteinization on samples if necessary.
	Sample readings exceed the linear range.	Concentrate or dilute the sample to keep readings within the linear range.
	Air bubbles in the wells.	Air bubbles will interfere with readings. Avoid bubble formation during operation and be sure to remove air bubbles before taking readings.
Low/high readings for samples and standards	Samples left for too long or stored improperly.	Use freshly prepared samples and store at the recommended temperature until use.
	Improper storage of kit components.	Store the kit as instructed in the manual.
	Inappropriate extraction time / temperature.	Refer to the extraction time and/or temperature recommended in the instructions.
	Incorrect reagent pipetting volume.	Check if the pipette is calibrated (always use the smallest-range pipette that can aspirate the full volume); avoid aspirating excessively small volumes of reagents as much as possible.
	Pipetting errors during reaction mixture preparation.	Always prepare a master mix.

规格

规格或纯度

BioReagent, 标曲法

英文名称

Soil Ammonium Nitrogen Content Assay Kit (Indophenol Blue, Colorimetric Method)

样品类型

土壤

检测方法

分光光度法

检测仪器

可见光分光光度计

检测波长

625 nm

敏感性

Light-sensitive

储存与运输

储存条件

2-8°C储存,避光

运输条件

低温运输

稳定性与储存

Store at 2-8℃ long term (6 months).

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
ZJ26F0939502	分析证书	S1527372

技术文档和文章

s1527372 土壤铵态氮含量检测试剂盒说明书.pdf

s1527372 soil ammonium nitrogen content assay kit indophenol blue colorimetric method manual.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 BioReagent 级别指南 → 查看 Standard Curve Method 级别指南 →

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。

土壤铵态氮含量检测试剂盒 (靛酚蓝, 比色法)

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

需要帮助选择级别？

查看相关“主题”系列

相关产品