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土壤多酚氧化酶（Polyphenoloxidase，PPO）是一类以铜、锰为活性中心的氧化还原酶，主要来源于土壤微生物、植物根系分泌物及动植物残体分解释放的酶。该酶能够将土壤中芳香族化合物氧化为醌，醌与土壤中蛋白质、氨基酸、糖类及矿物等物质发生反应，生成不同分子量的有机质和色素，完成土壤芳香族化合物的循环，同时亦可用于土壤环境修复。本试剂盒以左旋多巴为底物，PPO催化底物氧化生成红色醌类产物，该产物在475 nm处具有特征吸收峰，通过测定该波长下吸光值的增幅，即可精准计算土壤样品中PPO的酶活力大小。
Soil polyphenol oxidase (PPO) is a class of oxidoreductases with copper and manganese as the active centers. It is mainly derived from soil microorganisms, plant root exudates, and enzymes released during the decomposition of animal and plant residues. This enzyme is capable of oxidizing aromatic compounds in the soil to quinones, which further react with substances such as proteins, amino acids, carbohydrates, and minerals in the soil to generate organic matter and pigments of various molecular weights, thereby completing the cycling of aromatic compounds in the soil. It can also be applied in soil environmental remediation. This kit employs L-DOPA as the substrate, and PPO catalyzes the oxidation of the substrate to generate a red quinone product, which exhibits a characteristic absorption peak at 475 nm. By measuring the increase in absorbance at this wavelength, the PPO enzyme activity in soil samples can be accurately calculated.
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