计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤多糖是土壤有机质研究的重要组成部分。大量研究证实,土壤多糖对促进水稳性团聚体的形成、增强土壤结构稳定性、提高土壤抗侵蚀能力及保肥保水能力具有重要作用,因此土壤多糖含量的测定具有重要的研究意义。本试剂盒采用苯酚-硫酸比色法测定土壤多糖含量:土壤样品中的多糖在浓硫酸作用下水解生成单糖,单糖迅速脱水生成糠醛衍生物,糠醛衍生物与苯酚缩合生成橙黄色化合物,该产物颜色稳定,在488 nm处具有特征吸收峰,且在一定浓度范围内其吸光值与多糖含量呈线性正比关系。通过测定该波长下吸光值,结合标准曲线即可精准定量样品中的土壤多糖含量。
Soil polysaccharides are an important component of soil organic matter research. Numerous studies have demonstrated that soil polysaccharides play a crucial role in promoting the formation of water‑stable aggregates, enhancing soil structural stability, improving soil erosion resistance, and increasing soil water and nutrient retention capacity. Therefore, the determination of soil polysaccharide content is of significant research importance. This kit employs the phenol‑sulfuric acid colorimetric method for the determination of soil polysaccharide content. The principle is as follows: Polysaccharides in soil samples are hydrolyzed to monosaccharides under the action of concentrated sulfuric acid. The monosaccharides are rapidly dehydrated to generate furfural derivatives, which then condense with phenol to form an orange‑yellow compound. This product is stable in color and exhibits a characteristic absorption peak at 488 nm, with its absorbance showing a linear positive correlation with polysaccharide content within a certain concentration range. By measuring the absorbance at this wavelength and referring to a standard curve, the soil polysaccharide content in the sample can be accurately quantified.