BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酰胺酶（GLS，EC 3.5.1.2）是一种酰胺酶，催化谷氨酰胺水解生成谷氨酸和氨，在氮素代谢中具有重要调控作用。土壤谷氨酰胺酶（S-GLS）催化谷氨酰胺水解生成L-谷氨酸和氨（NH₃/NH₄⁺）。本试剂盒采用靛酚蓝比色法测定S-GLS活性：在强碱性条件下，氨与次氯酸盐和苯酚反应，生成稳定的水溶性蓝色靛酚蓝染料，该产物在630 nm处具有特征吸收峰。通过测定单位时间内吸光值的增幅，即可反映土壤样品中S-GLS的酶活性大小。
Glutaminase (GLS, EC 3.5.1.2) is an amidase that catalyzes the hydrolysis of glutamine to glutamate and ammonia, playing an important regulatory role in nitrogen metabolism. Soil glutaminase (S‑GLS) catalyzes the hydrolysis of glutamine to L‑glutamate and ammonia (NH₃/NH₄⁺). This kit employs the indophenol blue colorimetric method for the determination of S‑GLS activity. Under strongly alkaline conditions, ammonia reacts with hypochlorite and phenol to form a stable, water‑soluble blue indophenol blue dye, which exhibits a characteristic absorption peak at 630 nm. By measuring the increase in absorbance per unit time, the S‑GLS enzyme activity in the soil sample can be quantitatively reflected.
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