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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤过氧化物酶(S-POD)主要来源于土壤微生物,能够氧化土壤有机物质生成过氧化物,在腐殖质形成过程中发挥重要作用。该酶在过氧化氢存在下催化底物左旋多巴氧化,生成红色醌类产物。参考相关文献,选定该有色产物在475 nm处的特征吸收峰进行测定,通过吸光值变化计算土壤过氧化物酶活性。
Soil peroxidase (S‑POD) is primarily derived from soil microorganisms. It catalyzes the oxidation of soil organic matter to generate peroxides and plays an important role in the formation of humus. In the presence of hydrogen peroxide, this enzyme catalyzes the oxidation of the substrate L‑DOPA to form red quinone products. Based on relevant literature, the characteristic absorption peak of this colored product at 475 nm is selected for measurement, and the soil peroxidase activity is calculated from the change in absorbance.