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几丁质主要存在于虾、蟹、昆虫等甲壳类动物的外壳、软体动物的器官（如乌贼的软骨）及真菌的细胞壁中。几丁质酶（EC 3.2.1.14）可催化几丁质水解，具有抵御真菌侵染的作用，已成为抗真菌病害研究的热点之一。本试剂盒的测定原理为：几丁质酶水解几丁质生成N-乙酰氨基葡萄糖，N-乙酰氨基葡萄糖进一步与对二甲氨基苯甲醛反应生成红色化合物，该产物在585 nm处具有特征吸收峰。通过测定585 nm处吸光度的增加速率，即可反映几丁质酶的活性。
Chitin is primarily found in the exoskeletons of crustaceans such as shrimp, crabs, and insects, in the organs of mollusks (e.g., squid cartilage), and in the cell walls of fungi. Chitinase (EC 3.2.1.14) catalyzes the hydrolysis of chitin and plays a role in defending against fungal infection, making it a research hotspot in the study of antifungal diseases. The assay principle of this kit is as follows: Chitinase hydrolyzes chitin to produce N‑acetylglucosamine, which further reacts with p‑dimethylaminobenzaldehyde to form a red‑colored compound. This product exhibits a characteristic absorption peak at 585 nm. By measuring the rate of increase in absorbance at 585 nm, the activity of chitinase can be determined.
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