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锰过氧化物酶（MnP，EC 1.11.1.13）是真菌分泌的一种含亚铁血红素的糖基化胞外蛋白，主要存在于白腐菌和土壤枯草菌等担子真菌中，属于木质素降解酶系，能有效降解木质素及废水、环境中的多种抗性物质，如腐殖质酸和多环芳烃等有机污染物。土壤锰过氧化物酶（S-MnP）在Mn²⁺存在下催化底物二甲氧基苯酚（DMP）氧化，生成在470 nm处具有特征吸收峰的产物，通过监测该产物在470 nm处的生成速率即可计算MnP活性。
Manganese peroxidase (MnP, EC 1.11.1.13) is a glycosylated extracellular hemoprotein secreted by fungi, primarily found in basidiomycetes such as white‑rot fungi and soil‑inhabiting basidiomycetes. It belongs to the lignin‑degrading enzyme system and can effectively degrade lignin as well as various recalcitrant substances in wastewater and the environment, including humic acids and polycyclic aromatic hydrocarbons (PAHs). Soil manganese peroxidase (S‑MnP) catalyzes the oxidation of the substrate dimethoxyphenol (DMP) in the presence of Mn²⁺, generating a product with a characteristic absorption peak at 470 nm. By monitoring the rate of formation of this product at 470 nm, the MnP activity can be calculated.
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