计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤磷酸酶在土壤磷素循环中发挥关键作用,其活性是评价土壤磷素生物转化方向与强度的重要指标,并与土壤碳、氮含量、有效磷水平及pH值密切相关。 本试剂盒提供一种简单、灵敏、快速的土壤酸性磷酸酶(S-ACP)活力测定方法,基于PNPP比色法。其原理为:在酸性反应条件下,S-ACP催化底物磷酸对硝基苯酯(PNPP)水解,生成黄色对硝基苯酚(PNP);反应终止后(加入碱性溶液使PNP充分显色),PNP在405 nm处具有特征吸收峰。通过连续监测405 nm处PNP吸光度的上升速率(其与S-ACP活力成正比),即可定量计算样品中土壤酸性磷酸酶的活性。
Soil phosphatases play a critical role in soil phosphorus cycling, and their activity is an important indicator for evaluating the direction and intensity of phosphorus biotransformation in soil. It is closely correlated with soil carbon and nitrogen content, available phosphorus levels, and pH. This kit provides a simple, sensitive, and rapid method for determining soil acid phosphatase (S‑ACP) activity based on the PNPP colorimetric method. The principle is as follows: Under acidic reaction conditions, S‑ACP catalyzes the hydrolysis of the substrate p‑nitrophenyl phosphate (PNPP) to generate yellow p‑nitrophenol (PNP). After the reaction is terminated (by adding an alkaline solution to fully develop the PNP color), PNP exhibits a characteristic absorption peak at 405 nm. By continuously monitoring the rate of increase in absorbance at 405 nm, which is directly proportional to the S‑ACP activity, the soil acid phosphatase activity in the sample can be quantitatively calculated.