计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
木聚糖酶(EC3.2.1.8)主要由微生物产生,能催化水解木聚糖,也被称为戊聚糖酶或半纤维素酶,可分解酿造或饲料工业中的原料细胞壁以及β-葡聚糖,降低酿造中物料的粘度,促进有效物质的释放,以及降低饲料中的非淀粉多糖,促进营养物质的吸收利用,因而广泛的应用于酿造和饲料工业中,ACX 一般分离自耐酸的真菌,细菌及部分霉菌。该产品提供了一种简单、方便、快速的酸性木聚糖酶活性检测方法,适用于土壤样本。其原理是ACX在酸性环境下能将木聚糖降解成还原性寡糖和单糖,进一步在沸水浴条件下与 3,5-二硝基水杨酸发生显色反应,在540 nm处有特征吸收峰,反应液颜色的深浅与酶解产生的还原糖量成正比,通过测定反应液在 540 nm 吸光值增加速率,可计算ACX 活力。
Xylanase (EC 3.2.1.8) is mainly produced by microorganisms and catalyzes the hydrolysis of xylan. It is also known as pentosanase or hemicellulase. This enzyme can degrade the cell walls and β‑glucan in raw materials used in the brewing and feed industries, reducing the viscosity of the mash during brewing, promoting the release of active substances, and reducing non‑starch polysaccharides in feed, thereby facilitating nutrient absorption and utilization. Hence, it is widely applied in the brewing and feed industries. Acid xylanase (ACX) is generally isolated from acid‑tolerant fungi, bacteria, and some molds. This product provides a simple, convenient, and rapid method for determining acid xylanase activity, suitable for soil samples. The principle is that ACX degrades xylan into reducing oligosaccharides and monosaccharides under acidic conditions. These reducing sugars then react with 3,5‑dinitrosalicylic acid (DNS) in a boiling water bath, producing a colored product with a characteristic absorption peak at 540 nm. The color intensity of the reaction solution is directly proportional to the amount of reducing sugars generated by enzymatic hydrolysis. By measuring the rate of increase in absorbance at 540 nm, the ACX activity can be calculated.