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土壤脱氢酶活性可反映土壤中活性微生物的数量及其对有机污染物的降解能力，是评价土壤生物降解性能的重要指标。传统测定方法采用氯化三苯基四氮唑（TTC）作为氢受体，但其还原产物甲臜不溶于水，需有机溶剂萃取，操作繁琐且灵敏度较低。 本试剂盒提供一种简单、灵敏且快速的土壤脱氢酶活力测定方法。该方法采用改性水溶性四唑盐替代TTC作为氢受体，酶促还原生成的有色甲臜产物可直接溶于水，无需萃取步骤。该甲臜产物在460 nm处具有特征吸收峰，通过测定460 nm处反应液的吸光度（其与脱氢酶活性成正比），即可快速定量土壤脱氢酶活性。
Soil dehydrogenase activity reflects the number of active microorganisms in soil and their capability to degrade organic pollutants, and serves as an important indicator for evaluating soil biodegradability. The traditional assay method employs 2,3,5‑triphenyltetrazolium chloride (TTC) as a hydrogen acceptor; however, its reduction product formazan is water‑insoluble and requires organic solvent extraction, resulting in cumbersome operation and relatively low sensitivity. This kit provides a simple, sensitive, and rapid method for the determination of soil dehydrogenase activity. This method employs a modified water‑soluble tetrazolium salt as a hydrogen acceptor in place of TTC. The colored formazan product generated by enzymatic reduction is directly water‑soluble, eliminating the need for an extraction step. This formazan product exhibits a characteristic absorption peak at 460 nm. By measuring the absorbance of the reaction solution at 460 nm—which is directly proportional to the dehydrogenase activity—the soil dehydrogenase activity can be rapidly quantified.
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