计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用PNP比色法测定土壤外切-β-1,4-葡聚糖酶(S-CBH)活力。CBH(EC 3.2.1.91)是土壤纤维素酶系的重要组分,水解β-1,4-糖苷键释放纤维二糖。该酶催化底物对硝基苯基-β-D-纤维二糖苷生成黄色PNP产物,于405 nm处测定吸光度,吸光度与酶活力成正比,据此定量S-CBH活性。
This kit adopts the PNP colorimetric method to determine the activity of soil exo-β-1,4-glucanase (S-CBH). CBH (EC 3.2.1.91) is a vital component of the soil cellulase system, which hydrolyzes β-1,4-glycosidic bonds to release cellobiose. This enzyme catalyzes the substrate p-nitrophenyl-β-D-cellobioside to produce yellow PNP products. The absorbance is measured at 405 nm, and the absorbance value is proportional to enzyme activity, based on which the activity of S-CBH can be quantified.