BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤纤维素酶（S-CL）主要来源于土壤微生物，能够催化纤维素降解生成葡萄糖，是土壤碳循环中的关键酶之一。其降解农作物秸秆产生的葡萄糖，是土壤微生物生长代谢的重要碳源营养物质。本试剂盒采用蒽酮比色法测定S-CL的酶活性：S-CL催化纤维素（或羧甲基纤维素钠）水解生成还原糖；还原糖在浓硫酸作用下脱水生成糠醛类化合物，该化合物与蒽酮试剂反应生成蓝绿色产物，在620 nm处具有特征吸收峰。通过测定620 nm处吸光度的增加速率，即可定量计算S-CL的酶活性。
Soil cellulase (S-CL) is mainly derived from soil microorganisms. It catalyzes the degradation of cellulose to produce glucose and acts as one of the key enzymes involved in soil carbon cycling. Glucose generated from the enzymatic breakdown of crop straw serves as an important carbon nutrient source for the growth and metabolism of soil microbes. This kit uses the anthrone colorimetric method to measure S-CL activity: S-CL catalyzes the hydrolysis of cellulose (or sodium carboxymethyl cellulose) to yield reducing sugars. Under concentrated sulfuric acid, reducing sugars undergo dehydration to form furan compounds, which react with anthrone reagent to produce a blue-green product with a characteristic absorption peak at 620 nm. The enzymatic activity of S-CL can be quantitatively calculated by measuring the increasing rate of absorbance at 620 nm.
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