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血清总铁结合能力（TIBC）是反映血清转铁蛋白可结合铁能力的重要指标，其水平异常与缺铁性贫血、急性肝炎等疾病密切相关。本试剂盒采用菲洛嗪比色法测定TIBC：Fe²⁺与菲洛嗪反应生成紫红色化合物，该产物在562 nm处具有特征吸收峰。在碱性条件下，血清转铁蛋白与Fe³⁺结合，剩余未结合的Fe³⁺被还原为Fe²⁺，此时测得的吸光度A₁与未结合Fe³⁺量呈正比；酸化处理后，转铁蛋白结合的Fe³⁺被释放并还原为Fe²⁺，此时测得的吸光度A₂与总Fe³⁺量呈正比。A₂与A₁的差值（A₂−A₁）与TIBC浓度呈正比，通过测定该差值即可定量计算样品的总铁结合能力。
Serum total iron‑binding capacity (TIBC) is an important indicator reflecting the ability of serum transferrin to bind iron. Abnormal TIBC levels are closely associated with conditions such as iron deficiency anemia and acute hepatitis. This kit employs a ferrozine colorimetric method for the determination of TIBC. The principle is as follows: Fe²⁺ reacts with ferrozine to form a purple‑red compound, which exhibits a characteristic absorption peak at 562 nm. Under alkaline conditions, serum transferrin binds to Fe³⁺. The remaining unbound Fe³⁺ is reduced to Fe²⁺, and the measured absorbance A₁ is proportional to the amount of unbound Fe³⁺. After acidification treatment, the transferrin‑bound Fe³⁺ is released and reduced to Fe²⁺, and the measured absorbance A₂ is proportional to the amount of total Fe³⁺. The difference between A₂ and A₁ (A₂ − A₁) is proportional to the TIBC concentration. By measuring this difference, the total iron‑binding capacity of the sample can be quantitatively calculated.
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